製品の概要

  • 製品名Anti-MMP3 antibody
    MMP3 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to MMP3
  • 特異性ab18898 recognises MMP-3.
  • アプリケーション適用あり: IHC-Fr, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Horse, Human
  • 免疫原

    Recombinant full length protein (Human)(Peptide available as ab53328.)

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab18898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr
WB
IP
  • 追加情報IHC-Fr: Use at a concentration of 15 - 25 µg/ml.
    IP: Use at a concentration of 10 - 20 µg/ml.
    WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 54 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
    • 配列類似性Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 細胞内局在Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CHDS6 antibody
      • Matrix metalloproteinase 3 antibody
      • Matrix metalloproteinase-3 antibody
      • MGC126102 antibody
      • MGC126103 antibody
      • MGC126104 antibody
      • MMP 3 antibody
      • MMP-3 antibody
      • MMP3 antibody
      • MMP3_HUMAN antibody
      • Proteoglycanase antibody
      • SL-1 antibody
      • SL1 antibody
      • STMY antibody
      • STMY1 antibody
      • STR1 antibody
      • Stromelysin 1 antibody
      • Stromelysin-1 antibody
      • Transin 1 antibody
      • Transin-1 antibody
      see all

    Anti-MMP3 antibody 画像

    • Anti-MMP3 antibody (ab18898) at 1 µg/ml + Recombinant human MMP3 protein (ab96555) at 0.1 µg

      Secondary
      Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 3 minutes

    Anti-MMP3 antibody (ab18898) 使用論文

    This product has been referenced in:
    • Attia M  et al. Early Events of Overused Supraspinatus Tendons Involve Matrix Metalloproteinases and EMMPRIN/CD147 in the Absence of Inflammation. Am J Sports Med 41:908-17 (2013). WB ; Rat . Read more (PubMed: 23404084) »
    • Clutterbuck AL  et al. High throughput proteomic analysis of the secretome in an explant model of articular cartilage inflammation. J Proteomics 74:704-15 (2011). WB ; Horse . Read more (PubMed: 21354348) »

    See all 5 Publications for this product

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    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to co...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Horse Tissue lysate - other (Cartilage explant culture secretome)
    Loading amount 50 µg
    Specification Cartilage explant culture secretome
    Gel Running Conditions Reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Ali Mobasheri

    Verified customer

    投稿 Jan 19 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (Rat brain homogenate)
    Loading amount 60 µg
    Specification Rat brain homogenate
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Eduardo Candelario-Jalil

    Verified customer

    投稿 Jan 15 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"