製品の概要

  • 製品名Anti-MMP3 antibody [55-2A4]
    MMP3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [55-2A4] to MMP3
  • 特異性ab77962 specifically reacts with precursor and active forms of human MMP3. It cross reacts 34% with human MMP10.
  • アプリケーション適用あり: WB, IHC-Fr, IHC-P, ELISAmore details
  • 種交差性
    交差種: Rat, Sheep, Human, Pig
    非交差種: Mouse, Rabbit, Cow
  • 免疫原

    Full length native Human MMP3 protein (purified from synovial cells).

アプリケーション

Our Abpromise guarantee covers the use of ab77962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 54 kDa.
IHC-Fr Use a concentration of 0.5 µg/ml.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CHDS6 antibody
    • Matrix metalloproteinase 3 antibody
    • Matrix metalloproteinase-3 antibody
    • MGC126102 antibody
    • MGC126103 antibody
    • MGC126104 antibody
    • MMP 3 antibody
    • MMP-3 antibody
    • MMP3 antibody
    • MMP3_HUMAN antibody
    • Proteoglycanase antibody
    • SL-1 antibody
    • SL1 antibody
    • STMY antibody
    • STMY1 antibody
    • STR1 antibody
    • Stromelysin 1 antibody
    • Stromelysin-1 antibody
    • Transin 1 antibody
    • Transin-1 antibody
    see all

Anti-MMP3 antibody [55-2A4] 画像

  • IHC image of ab77962 staining in Hu Placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77962, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-MMP3 antibody [55-2A4] (ab77962) 使用論文

This product has been referenced in:
  • Cabuk H  et al. The effect of diclofenac on matrix metalloproteinase levels in the rotator cuff. Arch Orthop Trauma Surg 134:1739-44 (2014). Read more (PubMed: 25362529) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"