製品の概要

  • 製品名
    Anti-MMP3 antibody [4B7.3]
    MMP3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4B7.3] to MMP3
  • 特異性
    The antibody reacts with MMP3 (Stromelysin 1). It binds to both the reduced (the pro form as well as active forms) and the non reduced proteins. The antibody has been observerd to cross react with MMP10 (Stromelysin 2) but does not cross react with the other Stromelysin form (Stromelysin 3), nor to the other MMP family members (MMP1, MMP2, MMP9, etc.).
  • アプリケーション
    適用あり: WB, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein of Human MMP3.

  • ポジティブ・コントロール
    • Cell media from human chondrosarcoma (treated with TPA).
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 59, 57 kDa (predicted molecular weight: 54 kDa). Detects bands of approximately 59 and 57 kDa (the pro form) when used against the reduced protein. Dilution optimised using Chromogenic detection.
Sandwich ELISA Use a concentration of 1 µg/ml. For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab38912 as Detection.

ターゲット情報

  • 機能
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 配列類似性
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメイン
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CHDS6 antibody
    • Matrix metalloproteinase 3 antibody
    • Matrix metalloproteinase-3 antibody
    • MGC126102 antibody
    • MGC126103 antibody
    • MGC126104 antibody
    • MMP 3 antibody
    • MMP-3 antibody
    • MMP3 antibody
    • MMP3_HUMAN antibody
    • Proteoglycanase antibody
    • SL-1 antibody
    • SL1 antibody
    • STMY antibody
    • STMY1 antibody
    • STR1 antibody
    • Stromelysin 1 antibody
    • Stromelysin-1 antibody
    • Transin 1 antibody
    • Transin-1 antibody
    see all

画像

  • Standard curve for MMP3 (Analyte: ab39306) ; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [4B7.3] to MMP3 (ab38907) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP3 - Whole molecule (ab38912) at 0.5µg/ml.
  • All lanes : Anti-MMP3 antibody [4B7.3] (ab38907) at 1/1000 dilution

    Lane 1 : Human MMP3
    Lane 2 : Cell media from human chondrosarcoma (treated with TPA)


    Predicted band size : 54 kDa

参考文献

This product has been referenced in:
  • Beltrami-Moreira M  et al. Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling. PLoS One 11:e0152474 (2016). Read more (PubMed: 27032103) »
  • Sauls K  et al. Increased Infiltration of Extra-Cardiac Cells in Myxomatous Valve Disease. J Cardiovasc Dev Dis 2:200-213 (2015). IHC, ICC/IF ; Mouse . Read more (PubMed: 26473162) »

See all 6 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (Purified from cultured cells)
Loading amount
0.5 µg
Specification
Purified from cultured cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jul 05 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録