製品の概要

  • 製品名Anti-MMP21 antibody [EP1277Y]
    MMP21 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1277Y] to MMP21
  • 特異性ab52817 recognizes 2 isoforms of MMP21.
  • アプリケーション適用あり: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide that corresponds to residues from human MMP21.

  • ポジティブ・コントロール
    • Human kidney lysate and hepatocellular carcinoma tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50 - 1/100.
WB 1/200000. Detects a band of approximately 58,45 kDa (predicted molecular weight: 65 kDa).

This antibody recognizes 2 isoforms of MMP21, 58kDa and 45kDa bands are detected.

IP 1/30.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 関連性This protein is a member of the matrix metalloproteinase (MMP) family. MMP21 was first described as an MMP in Xenopus (XMMP), expressed in early embryo development. Later, species orthologs were reported in the Newt Cynops, and in rat, mouse and human. A literature alias was initiated by the deposit of sequences for MMP21/22, which were later renamed MMP23, the cysteine Array MMP (CAMMP). The mouse sequence seems also to be expressed early in embryo development, especially in the brain, and the human sequence has been reported in tumors. MMP21 contains the canonical MMP elements of cysteine switch (PRCGVPD) and HExGHxxxxxH catalytic zinc site, without a transmembrane sequence. The Xenopus and Cynops sequences contain a potential furin like cleavage site (RR/KKR), shortly after the cysteine switch, but the rat, mouse and human furin sites may not be functional. The hinge region is much shorter in MMP21 than in other MMPs, and the carboxyterminal half has some homology to vitronectin. Little is known about MMP21 function, expression or distribution. The relative low homology with other MMPs suggests a different distribution, and the regulatory elements known suggest quite different promotion than “classical” MMPs. The Xenopus and Cynops sequences have two inserts relative to the human and murine sequences, one in the propeptide domain, and one right after the cysteine switch. Interestingly, the pI for the human and murine proteins is quite basic, 9.334, 9.801 and 9.294 respectively for human, rat and mouse, while the Xenopus and Cynops predicted pI are 7.674 and 7.416. The basic pI and vitronectin homology suggest ECM localization. The 569 amino acid human zymogen has a predicted mass of 65.01 kD, the mouse and rat sequences at 65.45 and 61.07 kD, and the Xenopus and Cynops both about 70 kD, without posttranslational modifications.
  • 細胞内局在Secreted
  • 参照データベース
  • 別名
    • Matrix metalloproteinase 21 antibody
    • MIFR antibody
    • MMP 21 antibody

Anti-MMP21 antibody [EP1277Y] 画像

  • ab52817 at 1/100 dilution staining MMP21 in human hepatocellular carcinoma by Immunohistochemistry, Paraffin embedded tissue.
  • Anti-MMP21 antibody [EP1277Y] (ab52817) at 1/200000 dilution + human kidney lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 65 kDa
    Observed band size : 45,58 kDa (why is the actual band size different from the predicted?)

Anti-MMP21 antibody [EP1277Y] (ab52817) 使用論文

This product has been referenced in:
  • Ghosh MC  et al. CXCR4 regulates migration of lung alveolar epithelial cells through activation of Rac1 and matrix metalloproteinase-2. Am J Physiol Lung Cell Mol Physiol 302:L846-56 (2012). Read more (PubMed: 22345572) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"