製品の概要

  • 製品名Anti-MMP20 antibody
    MMP20 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MMP20
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the internal amino acids 396-445 (AAVYLREPQK TLFFVGDEYY SYDERKRKME KDYPKNTEEE FSGVNGQIDA) of Human MMP20 (NP_004762).

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab84737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation.
  • 組織特異性Expressed specifically in the enamel organ.
  • 関連疾患Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2) [MIM:612529]. AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel.
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾Autoactivates at least at the 107-Asn-
    -Tyr-108 site.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI2A2 antibody
    • Enamel metalloproteinase antibody
    • Enamelysin antibody
    • Matrix metalloproteinase 20 antibody
    • Matrix metalloproteinase-20 antibody
    • MMP 20 antibody
    • MMP-20 antibody
    • MMP20 antibody
    • MMP20_HUMAN antibody
    see all

Anti-MMP20 antibody 画像

  • Anti-MMP20 antibody (ab84737) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)

Anti-MMP20 antibody (ab84737) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab84737.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"