製品の概要

  • 製品名Anti-MMP2 antibody [EPR1184]
    MMP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1184] to MMP2
  • アプリケーション適用あり: ICC/IF, WB, Flow Cytmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MMP2 aa 550-650 (C terminal).
    Database link: P08253

  • ポジティブ・コントロール
    • L6, fetal heart and NIH/3T3 cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 74 kDa.
Flow Cyt 1/400.

For unpurified, use 1/70.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
      -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
      PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
    • 組織特異性Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
    • 関連疾患Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
    • 配列類似性Belongs to the peptidase M10A family.
      Contains 3 fibronectin type-II domains.
      Contains 4 hemopexin-like domains.
    • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻訳後修飾Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
      The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
    • 細胞内局在Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 72 kDa gelatinase antibody
      • 72kD type IV collagenase antibody
      • CLG 4 antibody
      • CLG 4A antibody
      • CLG4 antibody
      • CLG4A antibody
      • Collagenase Type 4 alpha antibody
      • Collagenase type IV A antibody
      • Gelatinase A antibody
      • Gelatinase alpha antibody
      • Gelatinase neutrophil antibody
      • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
      • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
      • Matrix Metalloproteinase 2 antibody
      • Matrix metalloproteinase II antibody
      • Matrix metalloproteinase-2 antibody
      • MMP 2 antibody
      • MMP II antibody
      • MMP-2 antibody
      • MMP2 antibody
      • MMP2_HUMAN antibody
      • MONA antibody
      • Neutrophil gelatinase antibody
      • PEX antibody
      • TBE 1 antibody
      • TBE-1 antibody
      see all

    Anti-MMP2 antibody [EPR1184] 画像

    • All lanes : Anti-MMP2 antibody [EPR1184] (ab92536) at 1/10000 dilution (purified)

      Lane 1 : L6 cell lysate
      Lane 2 : NIH/3T3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 74 kDa
      Observed band size : 64,72 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

      72kDa: propeptide; 64kDa: active form

    • Anti-MMP2 antibody [EPR1184] (ab92536) at 1/5000 dilution (purified) + human skin at 10 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 74 kDa
      Observed band size : 64,72 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

      72kDa: propeptide; 64kDa: active form

    • Immunofluorescence staining of PC-3 cells with purified ab92536 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab92536 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab92536 at a dilution of 1 in 400 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
    • All lanes : Anti-MMP2 antibody [EPR1184] (ab92536) at 1/1000 dilution (unpurified)

      Lane 1 : L6 cell lysate
      Lane 2 : Fetal heart lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 74 kDa

    Anti-MMP2 antibody [EPR1184] (ab92536) 使用論文

    This product has been referenced in:
    • Duan Y  et al. Targeted silencing of CXCR4 inhibits epithelial-mesenchymal transition in oral squamous cell carcinoma. Oncol Lett 12:2055-2061 (2016). WB . Read more (PubMed: 27602138) »
    • She S  et al. C-reactive protein is a biomarker of AFP-negative HBV-related hepatocellular carcinoma. Int J Oncol 47:543-54 (2015). Read more (PubMed: 26058824) »

    See all 8 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (Vascular smooth muscle cells)
    Gel Running Conditions Reduced Denaturing
    Loading amount 25 µg
    Specification Vascular smooth muscle cells
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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    投稿 Oct 10 2016

    Thank you for contacting us.

    I have had a chance to review the images which you sent to me. It looks to me that each product appears to be near the correct size however non specific bands and background makes determining the whether each i...

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