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RabMAb

Anti-MMP2 抗体 [EPR1184] (ab92536)

製品の概要

  • 製品名
    Anti-MMP2 antibody [EPR1184]
    MMP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1184] to MMP2
  • アプリケーション
    適用あり: ICC/IF, WB, Flow Cytmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MMP2 aa 550-650 (C terminal).
    Database link: P08253

  • ポジティブ・コントロール
    • L6, fetal heart and NIH/3T3 cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 74 kDa.
Flow Cyt 1/400.

For unpurified, use 1/70.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
      -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
      PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
    • 組織特異性
      Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
    • 関連疾患
      Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
    • 配列類似性
      Belongs to the peptidase M10A family.
      Contains 3 fibronectin type-II domains.
      Contains 4 hemopexin-like domains.
    • ドメイン
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻訳後修飾
      Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
      The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
    • 細胞内局在
      Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 72 kDa gelatinase antibody
      • 72kD type IV collagenase antibody
      • CLG 4 antibody
      • CLG 4A antibody
      • CLG4 antibody
      • CLG4A antibody
      • Collagenase Type 4 alpha antibody
      • Collagenase type IV A antibody
      • Gelatinase A antibody
      • Gelatinase alpha antibody
      • Gelatinase neutrophil antibody
      • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
      • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
      • Matrix Metalloproteinase 2 antibody
      • Matrix metalloproteinase II antibody
      • Matrix metalloproteinase-2 antibody
      • MMP 2 antibody
      • MMP II antibody
      • MMP-2 antibody
      • MMP2 antibody
      • MMP2_HUMAN antibody
      • MONA antibody
      • Neutrophil gelatinase antibody
      • PEX antibody
      • TBE 1 antibody
      • TBE-1 antibody
      see all

    画像

    • Immunofluorescence staining of PC-3 cells with purified ab92536 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab92536 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Anti-MMP2 antibody [EPR1184] (ab92536) at 1/5000 dilution (purified) + human skin at 10 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 74 kDa
      Observed band size : 64,72 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

      72kDa: propeptide; 64kDa: active form

    • Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma) cells labeling MMP2 with purified ab92536 at 1/180 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • All lanes : Anti-MMP2 antibody [EPR1184] (ab92536) at 1/10000 dilution (purified)

      Lane 1 : L6 cell lysate
      Lane 2 : NIH/3T3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 74 kDa
      Observed band size : 64,72 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

      72kDa: propeptide; 64kDa: active form

    • Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab92536 at a dilution of 1 in 400 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
    • All lanes : Anti-MMP2 antibody [EPR1184] (ab92536) at 1/1000 dilution (unpurified)

      Lane 1 : L6 cell lysate
      Lane 2 : Fetal heart lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 74 kDa

    参考文献

    This product has been referenced in:
    • Zhai L  et al. Picroside II protects the blood-brain barrier by inhibiting the oxidative signaling pathway in cerebral ischemia-reperfusion injury. PLoS One 12:e0174414 (2017). WB ; Rat . Read more (PubMed: 28388666) »
    • Wang R  et al. Downregulation of FKBP14 by RNA interference inhibits the proliferation, adhesion and invasion of gastric cancer cells. Oncol Lett 13:2811-2816 (2017). WB ; Human . Read more (PubMed: 28454471) »

    See all 18 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    Vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 10 2016

    Thank you for contacting us.

    I have had a chance to review the images which you sent to me. It looks to me that each product appears to be near the correct size however non specific bands and background makes determining the whether each i...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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