製品の概要

  • 製品名Anti-MMP2 antibody
    MMP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MMP2
  • アプリケーション適用あり: IHC-P, IP, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Sheep, Rabbit, Cow, Pig, Xenopus laevis
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 193 and 545 of Human MMP2 (NP_004521).

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lysate,PC-3,U87-MG,mouse brain

法規制情報

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab97779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/1000.
IP 1/100 - 1/500.
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 74 kDa.

ターゲット情報

  • 機能Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • 組織特異性Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • 関連疾患Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • 細胞内局在Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 72 kDa gelatinase antibody
    • 72kD type IV collagenase antibody
    • CLG 4 antibody
    • CLG 4A antibody
    • CLG4 antibody
    • CLG4A antibody
    • Collagenase Type 4 alpha antibody
    • Collagenase type IV A antibody
    • Gelatinase A antibody
    • Gelatinase alpha antibody
    • Gelatinase neutrophil antibody
    • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
    • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 2 antibody
    • Matrix metalloproteinase II antibody
    • Matrix metalloproteinase-2 antibody
    • MMP 2 antibody
    • MMP II antibody
    • MMP-2 antibody
    • MMP2 antibody
    • MMP2_HUMAN antibody
    • MONA antibody
    • Neutrophil gelatinase antibody
    • PEX antibody
    • TBE 1 antibody
    • TBE-1 antibody
    see all

Anti-MMP2 antibody 画像

  • Anti-MMP2 antibody (ab97779) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 74 kDa
    7.5% SDS Page
  • All lanes : Anti-MMP2 antibody (ab97779) at 1/3000 dilution

    Lane 1 : PC-3
    Lane 2 : U87-MG
    Lane 3 : SK-N-SH

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 74 kDa
  • Immunofluorescent analysis of methanol-fixed HeLa (Human epithelial cells from cervix adenocarcinoma cell line) labeling MMP2 with ab97779 at 1/200 dilution.

  • Immunohistochemical analysis of Human endometrical carcinoma labeling MMP2 with ab97779 at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MMP2 with ab97779 at 1/500 dilution.

Anti-MMP2 antibody (ab97779) 使用論文

ab97779 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-15%)
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Nov 05 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Antigen retrieval step None
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
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投稿 Nov 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"