製品の概要
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製品名Anti-MMP2 antibody [6E3F8]
MMP2 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [6E3F8] to MMP2
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由来種Mouse
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アプリケーション適用あり: IHC-FoFr, WB, IHC-Pmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Recombinant fragment derived from within residues 380 - 655 of Human MMP2.
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ポジティブ･コントロール
- This antibody gave a positive signal in the following lysates: Human Ovary Tissue; Human Liver Tissue; Mouse Liver Tissue; Rat Spleen Tissue; A375 Whole Cell; NIH3T3 Whole Cell; PC12 Whole Cell.
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特記事項
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
精製度IgG fraction
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ポリ/モノモノクローナル
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クローン名6E3F8
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アイソタイプIgG2a
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軽鎖の種類kappa
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研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab86607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FoFr | 1/300. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa). | |
| IHC-P | Use a concentration of 1 - 5 µg/ml. |
ターゲット情報
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機能Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. -
組織特異性Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
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関連疾患Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
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配列類似性Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains. -
ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
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翻訳後修飾Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3. -
細胞内局在Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
- Information by UniProt
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参照データベース
- Entrez Gene: 4313 Human
- Entrez Gene: 17390 Mouse
- Entrez Gene: 81686 Rat
- Omim: 120360 Human
- SwissProt: P08253 Human
- SwissProt: P33434 Mouse
- SwissProt: P33436 Rat
- Unigene: 513617 Human
see all -
別名
- 72 kDa gelatinase antibody
- 72kD type IV collagenase antibody
- CLG 4 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (ab86607)](http://a.abcam.com/ps/products/86/ab86607/Images/MMP2-Primary-antibodies-ab86607-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (ab86607)IHC image of ab86607 staining in Human Breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86607, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (ab86607)](http://a.abcam.com/ps/products/86/ab86607/Images/ab86607-295183-ab86607PMID25661015.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (ab86607)Image from Sun H et al., J Cell Mol Med. 2015;19(4):836-49. Fig 6.; doi: 10.1111/jcmm.12521.Immunohistochemical (parraffin-embedded sections) staining for MMP-2 in atheroslerotic lesions of aortic root in from right to left Ad-MIFi, Ad-EGFP, NS control and non-DM control groups using ab86607 at a dilution of 1/200..
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![Western blot - Anti-MMP2 antibody [6E3F8] (ab86607)](http://a.abcam.com/ps/products/86/ab86607/Images/MMP2-Primary-antibodies-ab86607-11.jpg)
Western blot - Anti-MMP2 antibody [6E3F8] (ab86607)All lanes : Anti-MMP2 antibody [6E3F8] (ab86607) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : Spleen (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 75 kDa (why is the actual band size different from the predicted?)
Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
![Western blot - Anti-MMP2 antibody [6E3F8] (ab86607)](http://a.abcam.com/ps/products/86/ab86607/Images/ab86607-233353-ab86608AP9767964minds.jpg)
Western blot - Anti-MMP2 antibody [6E3F8] (ab86607)All lanes : Anti-MMP2 antibody [6E3F8] (ab86607) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : A375 (Human melanoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 75 kDa (why is the actual band size different from the predicted?)
Additional bands at: 100 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab86607 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-MMP2 antibody [6E3F8] (ab86607)](http://a.abcam.com/ps/products/86/ab86607/Images/MMP2-Primary-antibodies-ab86607-17.jpg)
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-MMP2 antibody [6E3F8] (ab86607)This image is courtesy of an abreview submitted by Sophie Pezet, Laboratoire de Neurobiologie, FranceIHC-FoFr image of MMP2 staining on rat hippocampal sections using ab86607 (1:300). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. hippocampus was then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
参考文献
This product has been referenced in:
- Kumosa LS et al. Gelatin promotes rapid restoration of the blood brain barrier after acute brain injury. Acta Biomater 65:137-149 (2018). Read more (PubMed: 29037893) »
- Shao B et al. The 3p14.2 tumour suppressor ADAMTS9 is inactivated by promoter CpG methylation and inhibits tumour cell growth in breast cancer. J Cell Mol Med 22:1257-1271 (2018). WB ; Human . Read more (PubMed: 29193730) »
レビューと Q&A
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