Recombinant fragment derived from within residues 380 - 655 of Human MMP2.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Our Abpromise guarantee covers the use of ab86607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-FoFr | 1/300. | |
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa). | |
IHC-P | Use a concentration of 1 - 5 µg/ml. |
Immunohistochemical (parraffin-embedded sections) staining for MMP-2 in atheroslerotic lesions of aortic root in from right to left Ad-MIFi, Ad-EGFP, NS control and non-DM control groups using ab86607 at a dilution of 1/200..
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab86607 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"