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Our Abpromise guarantee covers the use of ab2462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 19910495|
|WB||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
400 ng samples of recombinant human pro-enzyme (lane 1 = MMP-1; lane 2 = MMP-3; lane 3 = MMP-2; lane 4 = MMP-9) were subjected to SDS-PAGE, transferred to PVDF membrane, and detected by immunoblotting with ab2462 specific for MMP-2.
IHC of human oesophageal adenocarcinoma labelling MMP2 with ab2462 (undiluted).
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