製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MMP19
  • 特異性
    ab53146 detects endogenous levels of total MMP19 protein.
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide derived from human MMP19.

  • ポジティブ・コントロール
    • WB: Extracts of HuvEc cells IHC-P: Human breast carcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 57 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

ターゲット情報

  • 機能
    Endopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin.
  • 組織特異性
    Expressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients.
  • 関連疾患
    May play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients.
  • 配列類似性
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメイン
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾
    Activated by autolytic cleavage after Lys-97.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Matrix metalloproteinase 18 antibody
    • Matrix metalloproteinase 19 antibody
    • Matrix metalloproteinase RASI antibody
    • Matrix metalloproteinase-18 antibody
    • Matrix metalloproteinase-19 antibody
    • MMP 18 antibody
    • MMP 19 antibody
    • MMP-18 antibody
    • MMP-19 antibody
    • MMP18 antibody
    • MMP19 antibody
    • MMP19_HUMAN antibody
    • RASI 1 antibody
    • RASI antibody
    • RASI1 antibody
    see all

画像

  • All lanes : Anti-MMP19 antibody (ab53146) at 1/500 dilution

    Lane 1 : Extracts of Huv Ec cells with no peptide
    Lane 2 : Extracts of Huv Ec cells with peptide


    Predicted band size : 57 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53146 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.

参考文献

This product has been referenced in:
  • Liu RY  et al. Overexpression of asparagine synthetase and matrix metalloproteinase 19 confers cisplatin sensitivity in nasopharyngeal carcinoma cells. Mol Cancer Ther 12:2157-66 (2013). Human . Read more (PubMed: 23956056) »
  • Wang L  et al. Co-expression of MMP-14 and MMP-19 predicts poor survival in human glioma. Clin Transl Oncol 15:139-45 (2013). Read more (PubMed: 22855183) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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