製品の概要
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製品名Anti-MMP19 antibody
MMP19 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to MMP19
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由来種Rabbit
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特異性ab53146 detects endogenous levels of total MMP19 protein.
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アプリケーション適用あり: WB, IHC-P, ELISAmore details
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種交差性交差種: Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide derived from human MMP19.
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ポジティブ･コントロール
- WB: Extracts of HuvEc cells IHC-P: Human breast carcinoma tissue
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab53146 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab53146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 57 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/10000. |
ターゲット情報
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機能Endopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin.
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組織特異性Expressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients.
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関連疾患May play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients.
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配列類似性Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
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翻訳後修飾Activated by autolytic cleavage after Lys-97.
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細胞内局在Secreted > extracellular space > extracellular matrix.
- Information by UniProt
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参照データベース
- Entrez Gene: 4327 Human
- Entrez Gene: 304608 Rat
- Omim: 601807 Human
- SwissProt: Q99542 Human
- Unigene: 591033 Human
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別名
- Matrix metalloproteinase 18 antibody
- Matrix metalloproteinase 19 antibody
- Matrix metalloproteinase RASI antibody
see all
画像
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Western blot - MMP19 antibody (ab53146)All lanes : Anti-MMP19 antibody (ab53146) at 1/500 dilution
Lane 1 : Extracts of Huv Ec cells with no peptide
Lane 2 : Extracts of Huv Ec cells with peptide
Predicted band size: 57 kDa
Observed band size: 62 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Paraffin-embedded sections) - MMP19 antibody (ab53146)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53146 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.
参考文献
This product has been referenced in:
- Liu RY et al. Overexpression of asparagine synthetase and matrix metalloproteinase 19 confers cisplatin sensitivity in nasopharyngeal carcinoma cells. Mol Cancer Ther 12:2157-66 (2013). Human . Read more (PubMed: 23956056) »
- Wang L et al. Co-expression of MMP-14 and MMP-19 predicts poor survival in human glioma. Clin Transl Oncol 15:139-45 (2013). Read more (PubMed: 22855183) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"