Anti-MMP16 抗体 (ab73877)
Key features and details
- Rabbit polyclonal to MMP16
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MMP16 antibody
MMP16 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MMP16 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IHC-Frmore details -
種交差性
交差種: Rat, Human -
免疫原
A synthetic peptide mapping at the C-terminal of human MMP16.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73877の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
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IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. |
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. |
ターゲット情報
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機能
Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells. -
組織特異性
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary. -
配列類似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
発生段階
Expressed in tissues undergoing reconstruction. Present in fetal tissues, especially in brain. Expression seems to decline with advanced development. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
The precursor is cleaved by a furin endopeptidase. -
細胞内局在
Cell membrane. Localized at the cell surface of melanoma cells and Secreted > extracellular space > extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells. - Information by UniProt
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参照データベース
- Entrez Gene: 4325 Human
- Entrez Gene: 65205 Rat
- Omim: 602262 Human
- SwissProt: P51512 Human
- SwissProt: O35548 Rat
- Unigene: 492187 Human
- Unigene: 546267 Human
- Unigene: 208361 Rat
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別名
- C8orf57 antibody
- DKFZp761D112 antibody
- Matrix metalloproteinase 16 antibody
see all
画像
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All lanes : Anti-MMP16 antibody (ab73877) at 1 µg/ml
Lane 1 : Whole cell lysates prepared from Human Hela cells.
Lane 2 : Whole cell lysates prepared from Human Jurkat cells.
Lane 3 : Whole cell lysates prepared from Human Colo320 cells.
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution
Predicted band size: 57 kDa -
Immunohistochemical analysis of paraffin-embedded sections Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml.
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Immunohistochemical analysis of frozen sections Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab73877 は 13 報の論文で使用されています。
- Schniers BK et al. PEPT1 is essential for the growth of pancreatic cancer cells: a viable drug target. Biochem J 478:3757-3774 (2021). PubMed: 34569600
- Zhou H & Chen Y CircRNA has_circ_0001806 promotes hepatocellular carcinoma progression via the miR-193a-5p/MMP16 pathway. Braz J Med Biol Res 54:e11459 (2021). PubMed: 34730679
- Zhang Y et al. Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs. Bioengineered 12:12767-12777 (2021). PubMed: 34747300
- Xu E et al. Exogenous HMGN2 inhibits the migration and invasion of osteosarcoma cell lines. Transl Cancer Res 9:1795-1805 (2020). PubMed: 35117527
- Zheng W et al. microRNA-26a Directly Targeting MMP14 and MMP16 Inhibits the Cancer Cell Proliferation, Migration and Invasion in Cutaneous Squamous Cell Carcinoma. Cancer Manag Res 12:7087-7095 (2020). PubMed: 32848463