製品の概要

  • 製品名Anti-MMP14 antibody [LEM-2/63.1]
    MMP14 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [LEM-2/63.1] to MMP14
  • アプリケーション適用あり: WB, IP, ELISA, IHC-Fr, Flow Cyt, Inhibition Assay, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Xenopus laevis, Macaque Monkey
  • 免疫原

    Synthetic peptide: C-AEPWTVRNEDLNGNDI-C, corresponding to amino acids 218-233 within the catalytic domain of Human MMP14

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Inhibition Assay Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
  • 組織特異性Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾The precursor is cleaved by a furin endopeptidase.
  • 細胞内局在Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Matrix metallopeptidase 14 (membrane inserted) antibody
    • Matrix metalloproteinase 14 antibody
    • Matrix metalloproteinase-14 antibody
    • Membrane type 1 matrix metalloproteinase antibody
    • Membrane type 1 metalloprotease antibody
    • Membrane type matrix metalloproteinase 1 antibody
    • Membrane-type matrix metalloproteinase 1 antibody
    • Membrane-type-1 matrix metalloproteinase antibody
    • MMP 14 antibody
    • MMP X1 antibody
    • MMP-14 antibody
    • MMP-X1 antibody
    • Mmp14 antibody
    • MMP14_HUMAN antibody
    • MMPX1 antibody
    • MT MMP 1 antibody
    • MT-MMP 1 antibody
    • MT1 MMP antibody
    • MT1-MMP antibody
    • MT1MMP antibody
    • MTMMP 1 antibody
    • MTMMP1 antibody
    see all

Anti-MMP14 antibody [LEM-2/63.1] (ab78738) 使用論文

This product has been referenced in:
  • Wali N  et al. Centrosomal BRCA2 is a target protein of membrane type-1 matrix metalloproteinase (MT1-MMP). Biochem Biophys Res Commun 443:1148-54 (2014). Read more (PubMed: 24384087) »
  • Kaewprag J  et al. HPV16 oncoproteins promote cervical cancer invasiveness by upregulating specific matrix metalloproteinases. PLoS One 8:e71611 (2013). WB, Inhibition Assay ; Human . Read more (PubMed: 23967226) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us. The paper that first describes the inhibition of MMP14 activity by this clone is the Galvez 2001 reference listed towards the bottom of the online datasheet, in figure 5. It also describes production of the clone and ot...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"