アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Synthetic peptide corresponding to Human MMP14.
(Peptide available as
A trial size is available to purchase for this antibody.
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab51074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For unpurified use at 1/100.
|WB||1/5000. Predicted molecular weight: 66 kDa.Can be blocked with MMP14 peptide (ab185123).
For unpurified use at 1/2000.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium carcinoma tissue sections labeling MMP14 with purified ab51074 at 1/100 dilution (1.7 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
Blocking and diluting buffer: 5% NFDM/TBST.
Flow cytometry analysis of A549 (human lung carcinoma) cells labeling MMP14 (red) with ab51074 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
ab51074 (purified) at 1/20 dilution (2µg) immunoprecipitating MMP14 in A431 (human epidermoid carcinoma) whole cell lysate.
Lane 1 (input): A431 (human epidermoid carcinoma)whole cell lysate 10ug
Lane 2 (+): ab51074 + A431 (human epidermoid carcinoma)whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51074 in A431 (human epidermoid carcinoma) whole cell lysate
For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1000).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Blocking/Diluting buffer and concentration 5% NFDM/TBST
63 kDa: pro-form; 60 kDa: active form.
MCF-7 is a MMP-14 negative or weakly expressed cell line (PMID: 25977338 and PMID: 19208838).
ab51074 (unpurified) at 1/500 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step (in Tris/EDTA) was performed. The tissue was then blocked with serum and incubated with the primary antibody. A biotinylated donkey anti-rabbit IgG was used as the secondary.