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RabMAb

Anti-MMP14 抗体 [EP1264Y] (ab51074)

製品の概要

  • 製品名
    Anti-MMP14 antibody [EP1264Y]
    MMP14 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1264Y] to MMP14
  • アプリケーション
    適用あり: IHC-P, IP, WBmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow
  • 免疫原

    Synthetic peptide corresponding to Human MMP14.
    (Peptide available as ab185123)

  • ポジティブ・コントロール
    • IHC: Human breast carcinoma, endometrium carcinoma and kidney tissues. WB: Human fetal spleen, esophagus and lung cancer tissue lysates, mouse and rat spleen tissue lysates and A431 whole cell lysate. Flow Cyt: A549 cells. IP: A431 cell lysate.
  • 特記事項

    A trial size is available to purchase for this antibody.

    The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.
IP 1/20.

For unpurified use at 1/100.

WB 1/5000. Predicted molecular weight: 66 kDa.Can be blocked with MMP14 peptide (ab185123).

For unpurified use at 1/2000.

  • 追加情報
    Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能
      Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
    • 組織特異性
      Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
    • 配列類似性
      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • ドメイン
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻訳後修飾
      The precursor is cleaved by a furin endopeptidase.
    • 細胞内局在
      Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Matrix metallopeptidase 14 (membrane inserted) antibody
      • Matrix metalloproteinase 14 antibody
      • Matrix metalloproteinase-14 antibody
      • Membrane type 1 matrix metalloproteinase antibody
      • Membrane type 1 metalloprotease antibody
      • Membrane type matrix metalloproteinase 1 antibody
      • Membrane-type matrix metalloproteinase 1 antibody
      • Membrane-type-1 matrix metalloproteinase antibody
      • MMP 14 antibody
      • MMP X1 antibody
      • MMP-14 antibody
      • MMP-X1 antibody
      • Mmp14 antibody
      • MMP14_HUMAN antibody
      • MMPX1 antibody
      • MT MMP 1 antibody
      • MT-MMP 1 antibody
      • MT1 MMP antibody
      • MT1-MMP antibody
      • MT1MMP antibody
      • MTMMP 1 antibody
      • MTMMP1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium carcinoma tissue sections labeling MMP14 with purified ab51074 at 1/100 dilution (1.7 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    • All lanes : Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/5000 dilution (purified)

      Lane 1 : Human fetal spleen lysate
      Lane 2 : Human lung cancer lysate
      Lane 3 : Mouse spleen lysate
      Lane 4 : Rat spleen lysate
      Lane 5 : Human esophagus lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 66 kDa
      Observed band size : 66 kDa

      Blocking and diluting buffer: 5% NFDM/TBST.

    • ab51074 (purified) at 1/20 dilution (2µg) immunoprecipitating MMP14 in A431 (human epidermoid carcinoma) whole cell lysate.

      Lane 1 (input): A431 (human epidermoid carcinoma)whole cell lysate 10ug
      Lane 2 (+): ab51074 + A431 (human epidermoid carcinoma)whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51074 in A431 (human epidermoid carcinoma) whole cell lysate

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1000).

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • All lanes : Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/2000 dilution (unpurified)

      Lane 1 : Human fetal spleen tissue lysate
      Lane 2 : Human lung cancer tissue lysate
      Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 66 kDa
      Observed band size : 60,63 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 seconds

      Blocking/Diluting buffer and concentration 5% NFDM/TBST

      63 kDa: pro-form; 60 kDa: active form.

      MCF-7 is a MMP-14 negative or weakly expressed cell line (PMID: 25977338 and PMID: 19208838).

    • ab51074 (unpurified) at 1/500 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step (in Tris/EDTA) was performed. The tissue was then blocked with serum and incubated with the primary antibody. A biotinylated donkey anti-rabbit IgG was used as the secondary.

      See Abreview

    プロトコール

    参考文献

    This product has been referenced in:
    • Peláez R  et al. ß3 integrin expression is required for invadopodia-mediated ECM degradation in lung carcinoma cells. PLoS One 12:e0181579 (2017). Read more (PubMed: 28767724) »
    • Dourado KMC  et al. Endoglin: a novel target for therapeutic intervention in acute leukemias revealed in xenograft mouse models. Blood 129:2526-2536 (2017). Read more (PubMed: 28351936) »

    See all 52 Publications for this product

    レビューと Q&A

    Thank you for contacting us.


    According to our internal records, this immunogen isnot from the N-terminus. In addition, itwill recognise pro and mature forms.


    I hope this information is helpful to you. Please do not hesi...

    Read More
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 10 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Brain)
    Permeabilization
    Yes - 0.1% Triton-X in PBS
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Mr. Daniel Lustberg

    Verified customer

    投稿 May 25 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Bos taurus Tissue sections (Airway)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
    Permeabilization
    No
    Specification
    Airway
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Dec 23 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Mouse Tissue (frozen lung tissue)
    Specification
    frozen lung tissue
    Permeabilization
    Yes - triton
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 May 23 2014

    We do not have any data for blocking studies with the rabbit monoclonal antibody ab51074. Currently, the only MMP14 antibody that we know has been tested for inhibition studies (assuming this is what you mean by "blocking") is the mouse monoclonal anti...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Heart, Left Ventricle)
    Loading amount
    40 µg
    Specification
    Heart, Left Ventricle
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dawn Bowles

    Verified customer

    投稿 Dec 28 2012

    Thank you for contacting us and your interest in our products. As discussed over the phone, I have checked the concentration of the antibodies and lot numbers you provided. The concentrations are as follows:

    ab52897, GR5286-6, 0.165 mg/ml;
    Read More

    Thank you for your enquiry.

    I can confirm that the immunogen sequence for MMP14 antibody [EP1264Y] (ab51074) is derived from somewhere within amino acids 150-180 (extracellular region) of human MMP-14 which is in the extracellular domain. I ...

    Read More

    Thank you for your enquiry.

    I can confirm that ab51074 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on ...

    Read More

    1-10 of 21 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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