製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MMP13
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Human recombinant MMP13 protein.

  • ポジティブ・コントロール
    • Conditioned, serum-free medium from (TPA-treated) rat CCL-4 cells. Placenta tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 - 5 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 10 - 20 µg/ml.

ターゲット情報

  • 機能
    Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
  • 組織特異性
    Seems to be specific to breast carcinomas.
  • 関連疾患
    Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
    Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
  • 配列類似性
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメイン
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CLG 3 antibody
    • CLG3 antibody
    • Collagenase 3 antibody
    • Collagenase3 antibody
    • MANDP1 antibody
    • Matrix metallopeptidase 13 (collagenase 3) antibody
    • Matrix Metalloproteinase 13 antibody
    • Matrix metalloproteinase-13 antibody
    • MMP 13 antibody
    • MMP-13 antibody
    • Mmp13 antibody
    • MMP13_HUMAN antibody
    see all

画像

  • Anti-MMP13 antibody (ab75606) at 5 µg/ml + Conditioned, serum-free medium from (TPA-treated) rat CCL-4 cells

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • ab75606 staining MMP13 in Rat shoulder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked witha vector kit for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in antibody diluent) for 16 hours at 4°C. A undiluted Biotin-conjugated polyclonal was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Isaka S  et al. Evaluation of the effect of oral administration of collagen peptides on an experimental rat osteoarthritis model. Exp Ther Med 13:2699-2706 (2017). Read more (PubMed: 28587333) »
  • Monteforte R  et al. SNEV(Prp19/PSO4) deficiency increases PUVA-induced senescence in mouse skin. Exp Dermatol 25:212-7 (2016). WB . Read more (PubMed: 26663487) »

See all 22 Publications for this product

レビューと Q&A

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Application
Western blot
Sample
Mouse Tissue lysate - whole (Cerebellum Tumor Cell)
Loading amount
30 µg
Specification
Cerebellum Tumor Cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

投稿 Oct 31 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Shoulder)
Specification
Shoulder
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Vector Kit as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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