製品の概要

  • 製品名
  • 製品の詳細
    Chicken polyclonal to MMP12
  • 由来種
    Chicken
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Fusion protein, corresponding to amino acids 1-33 of Human MMP12

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab26832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Predicted molecular weight: 54 kDa.

ターゲット情報

  • 機能
    May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
  • 組織特異性
    Found in alveolar macrophages but not in peripheral blood monocytes.
  • 配列類似性
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメイン
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    The presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.
  • 別名
    • EC 3.4.24.65 antibody
    • HME antibody
    • Macrophage elastase antibody
    • Macrophage metalloelastase antibody
    • Macrophage metaloelastase antibody
    • Matrix metallopeptidase 12 (macrophage elastase) antibody
    • Matrix metalloprotease 12 antibody
    • Matrix metalloproteinase-12 antibody
    • ME antibody
    • MGC138506 antibody
    • MME antibody
    • MMP 12 antibody
    • MMP-12 antibody
    • Mmp12 antibody
    • MMP12_HUMAN antibody
    see all

画像

  • Anti-MMP12 antibody (ab26832) at 1/5000 dilution + human MMP12 protein 400ng (1-33amino acids)

    Secondary
    goat-anti-IgY/Fc-HRP

    Developed using the ECL technique.

    Predicted band size: 54 kDa
    Observed band size: 15 kDa (why is the actual band size different from the predicted?)



    Different expression length of fusion protein will result in different MW of MMP 12.

プロトコール

参考文献

This product has been referenced in:
  • Alarab M  et al. Expression of Extracellular Matrix-Remodeling Proteins Is Altered in Vaginal Tissue of Premenopausal Women With Severe Pelvic Organ Prolapse. Reprod Sci N/A:N/A (2013). WB, IHC-Fr ; Human . Read more (PubMed: 24343133) »

See 1 Publication for this product

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