製品の概要

  • 製品名Anti-MMP11 antibody [EP1259Y]
    MMP11 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1259Y] to MMP11
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of human MMP11.

  • ポジティブ・コントロール
    • MCF-7 cell lysate, human breast carcinoma tissue or MCF7 cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab52904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
WB 1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P Use at an assay dependent concentration.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能May play an important role in the progression of epithelial malignancies.
    • 組織特異性Specifically expressed in stromal cells of breast carcinomas.
    • 配列類似性Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻訳後修飾The precursor is cleaved by a furin endopeptidase.
    • 細胞内局在Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Matrix Metalloproteinase 11 antibody
      • Matrix metalloproteinase-11 antibody
      • MMP-11 antibody
      • Mmp11 antibody
      • MMP11_HUMAN antibody
      • SL 3 antibody
      • SL-3 antibody
      • SL3 antibody
      • ST3 antibody
      • STMY3 antibody
      • Stromelysin 3 antibody
      • Stromelysin III antibody
      • Stromelysin-3 antibody
      see all

    Anti-MMP11 antibody [EP1259Y] 画像

    • Anti-MMP11 antibody [EP1259Y] (ab52904) at 1/500 dilution + MCF-7 cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled. at 1/2000 dilution

      Predicted band size : 55 kDa
      Observed band size : 55 kDa
    • Ab52904 (1:100) staining human MMP11 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunofluorescent staining of MCF7 cells using ab52904 (1:100).

    Anti-MMP11 antibody [EP1259Y] (ab52904) 使用論文

    This product has been referenced in:
    • Yonemori M  et al. Dual tumor-suppressors miR-139-5p and miR-139-3p targeting matrix metalloprotease 11 in bladder cancer. Cancer Sci 107:1233-42 (2016). WB ; Human . Read more (PubMed: 27355528) »
    • Roscilli G  et al. Circulating MMP11 and specific antibody immune response in breast and prostate cancer patients. J Transl Med 12:54 (2014). IHC-P ; Human . Read more (PubMed: 24564996) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Human Tissue sections (Breast carcinoma)
    Specification Breast carcinoma
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Jun 12 2013

    Thank you for your telephone call earlier this afternoon. I am very pleased to hear you would like to accept our offer and test ab52904 in IHC-Fr.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"