Anti-MMP11 抗体 (ab119284)
Key features and details
- Rabbit polyclonal to MMP11
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MMP11 antibody
MMP11 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MMP11 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide conjugated to KLH, derived from internal sequence amino acids of Human MMP11.
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ポジティブ・コントロール
- Human placenta and spleen tissues; A549 cell lysate; A549 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+, Ca2+ -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab119284の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 55 kDa.
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IHC-P |
Use a concentration of 10 µg/ml.
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ICC/IF |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 55 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
May play an important role in the progression of epithelial malignancies. -
組織特異性
Specifically expressed in stromal cells of breast carcinomas. -
配列類似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
The precursor is cleaved by a furin endopeptidase. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 4320 Human
- Entrez Gene: 17385 Mouse
- Entrez Gene: 25481 Rat
- Omim: 185261 Human
- SwissProt: P24347 Human
- SwissProt: Q02853 Mouse
- SwissProt: Q499S5 Rat
- Unigene: 143751 Human
see all -
別名
- Matrix Metalloproteinase 11 antibody
- Matrix metalloproteinase-11 antibody
- MMP-11 antibody
see all
画像
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ab119284, at 10 µg/ml, staining MMP11 in Formalin-fixed, Paraffin-embedded Human placenta tissue by Immunohistochemistry.
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ab119284, at 10 µg/ml, staining MMP11 in Formalin-fixed, Paraffin-embedded Human spleen tissue by Immunohistochemistry.
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All lanes : Anti-MMP11 antibody (ab119284) at 1/500 dilution
Lane 1 : A549 cell lysate
Lane 2 : A549 cell lysate with synthesized peptide
Predicted band size: 55 kDa -
ab119284, at 1/100, staining MMP11 in A549 cells by Immunofluorescence. The image on the right shows cells treated with synthesised peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab119284 は 4 報の論文で使用されています。
- Yang Y et al. Lamin B1 is a potential therapeutic target and prognostic biomarker for hepatocellular carcinoma. Bioengineered 13:9211-9231 (2022). PubMed: 35436411
- Zhuang Y et al. MMP11 promotes the proliferation and progression of breast cancer through stabilizing Smad2 protein. Oncol Rep 45:N/A (2021). PubMed: 33649832
- Yang L et al. LINC00221 silencing prevents the progression of hepatocellular carcinoma through let-7a-5p-targeted inhibition of MMP11. Cancer Cell Int 21:202 (2021). PubMed: 33836753
- Lee J et al. Identification of Matrix Metalloproteinase 11 as a Prognostic Biomarker in Pancreatic Cancer. Anticancer Res 39:5963-5971 (2019). PubMed: 31704821