製品の概要

  • 製品名Anti-MMP1 antibody [SB12e]
    MMP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SB12e] to MMP1
  • 特異性ab25483 recognises MMP1. It does not react with MMP2, MMP3 or MMP9.
  • アプリケーション適用あり: WB, ELISA, IHC-Fr, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human): MMP1

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 5 µg/ml. For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab38924 as Detection.

ターゲット情報

  • 機能Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメインThere are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 27 kDa interstitial collagenase antibody
    • CLG antibody
    • CLGN antibody
    • collagenase, fibroblast antibody
    • collagenase, interstitial antibody
    • Fibroblast collagenase antibody
    • Interstitial collagenase antibody
    • Matrix metallopeptidase 1 (interstitial collagenase) antibody
    • Matrix metalloprotease 1 antibody
    • Matrix Metalloproteinase 1 antibody
    • Matrix metalloproteinase-1 antibody
    • MMP 1 antibody
    • MMP-1 antibody
    • MMP1 antibody
    • MMP1_HUMAN antibody
    • OTTHUMP00000045866 antibody
    see all

Anti-MMP1 antibody [SB12e] 画像

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Anti-MMP1 antibody [SB12e] (ab25483) 使用論文

This product has been referenced in:
  • Lama P  et al. Do intervertebral discs degenerate before they herniate, or after? Bone Joint J 95-B:1127-33 (2013). Read more (PubMed: 23908431) »
  • Fabre V  et al. Tissue inhibitor of matrix-metalloprotease-1 predicts risk of hepatic fibrosis in human Schistosoma japonicum infection. J Infect Dis 203:707-14 (2011). ELISA . Read more (PubMed: 21199883) »

See all 3 Publications for this product

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Application Western blot
Sample Rabbit Tissue lysate - whole (heart)
Loading amount 40 µg
Specification heart
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

投稿 Oct 13 2010

Thank you for your enquiry. It appears from the information on these antibodies that they have been tested and shown to work in ELISA, but they were all tested using an indirect ELISA only (antigen coated on the plate, then primary and secondary ant...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"