製品の概要

  • 製品名Anti-MMP1 antibody
    MMP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MMP1
  • 特異性This antibody binds to MMP1, but does not cross react with the other MMP family members (MMP2, MMP3, MMP9).
  • アプリケーション適用あり: IHC-P, ICC/IF, IHC-Fr, WBmore details
  • 種交差性
    交差種: Horse, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to MMP1. based on the hemopexin domain of the human sequence
    (Peptide available as ab41003)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 18434409
IHC-Fr Use at an assay dependent concentration.
WB 1/5000. Predicted molecular weight: 54 kDa. When used against the reduced protein this antibody identifies bands at 53 Kd and 51 Kd (the pro-form), as well as the initial active forms. Dilution optimised using Chromogenic detection.

ターゲット情報

  • 機能Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメインThere are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 27 kDa interstitial collagenase antibody
    • CLG antibody
    • CLGN antibody
    • collagenase, fibroblast antibody
    • collagenase, interstitial antibody
    • Fibroblast collagenase antibody
    • Interstitial collagenase antibody
    • Matrix metallopeptidase 1 (interstitial collagenase) antibody
    • Matrix metalloprotease 1 antibody
    • Matrix Metalloproteinase 1 antibody
    • Matrix metalloproteinase-1 antibody
    • MMP 1 antibody
    • MMP-1 antibody
    • MMP1 antibody
    • MMP1_HUMAN antibody
    • OTTHUMP00000045866 antibody
    see all

Anti-MMP1 antibody 画像

  • All lanes : Anti-MMP1 antibody (ab38929)

    Lane 1 : Human MMP1.
    Lane 2 : Cell Media from human chondrosarcoma (no treatment).
    Lane 3 : Cell Media from human chondrosarcoma (treated with TPA).


    Predicted band size : 54 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
  • ab47116 (2µg/ml) staining Zinc Alpha 2 Glycoprotein in human breast tumour using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining in Myoepithelial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

プロトコール

Anti-MMP1 antibody (ab38929) 使用論文

This product has been referenced in:
  • Ferreira DS  et al. Molecularly engineered self-assembling membranes for cell-mediated degradation. Adv Healthc Mater 4:602-12 (2015). Read more (PubMed: 25413155) »
  • Jiang Y  et al. Downregulation of SPARC expression inhibits the invasion of human trophoblast cells in vitro. PLoS One 8:e69079 (2013). WB . Read more (PubMed: 23935929) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A549)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 60 µg
Specification A549
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Apr 06 2016

The laboratory that produces this antibody reports that it is unlikely to cross-react with mouse MMP1:

"Mice make 2 forms of MMP-1, the A form (NP_114395.1) and the B form (NP_114396.3), although the proteins have long been thought to be mad...

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For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7)
Loading amount 100000 cells
Specification MCF7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 May 23 2012

Thank you for your enquiry. I can confirm that ab38929 MMP1 antibody is known to detect both pro and active forms of MMP1. I hope this is helpful to you. Should you have any further questions, please do not hesitate to contact us.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Horse Tissue lysate - other (Cartilage explant culture secretome)
Loading amount 50 µg
Specification Cartilage explant culture secretome
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Ali Mobasheri

Verified customer

投稿 Jan 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (human fetal heart)
Specification human fetal heart
Fixative Paraformaldehyde
Permeabilization Yes - saponin 0.1% in PBS
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 May 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Horse Cell (fibroblast)
Specification fibroblast
Fixative Formaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 0.1%
Username

Dr. Yuan ZhengQiang

Verified customer

投稿 Apr 03 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Horse Tissue sections (skin tumor)
Specification skin tumor
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 0.1%
Username

Abcam user community

Verified customer

投稿 Mar 30 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"