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Synthetic peptide corresponding to MMP1. based on the hemopexin domain of the human sequence
(Peptide available as
Our Abpromise guarantee covers the use of ab38929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 18434409|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/5000. Predicted molecular weight: 54 kDa. When used against the reduced protein this antibody identifies bands at 53 Kd and 51 Kd (the pro-form), as well as the initial active forms. Dilution optimised using Chromogenic detection.|
ab38929 staining MMP1 in Horse fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 0.1% serum for 30 minutes. Samples were incubated with primary antibody (1/100) for 1 hour. An undiluted FITC-conjugated Mouse anti-rabbit IgG polyclonal was used as the secondary antibody. F-actin stained red and nuclear stained in blue with DAPI
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