MMP-3 Activity Assay Kit (Fluorometric - Green) (ab112148)

製品の概要

  • 製品名MMP-3 Activity Assay Kit (Fluorometric - Green)
    MMP3 キット 製品一覧
  • サンプルの種類
    Cell culture extracts, Adherent cells, Suspension cells
  • アッセイタイプDirect
  • 製品の概要

    MMP-3 (Matrix Metalloproteinase-3), also known as stromelysin-1, is involved in wound repair, atherosclerosis and tumors. ab112148 MMP-3 Activity Assay Kit uses a Green Fluorescence probe/fluorescence resonance energy transfer (FRET) peptide as the MMP-3 activity indicator. In the intact FRET peptide, the fluorescence of one half of the probe is quenched by the other part. Upon cleavage into two separate fragments by MMP-3, the green fluorescence is recovered.

    ab112148 is designed to monitor the activity of an MMP-3 enzyme. The peptide sequence used in the kit is more selective for MMP-3 hydrolysis than other MMP enzymes. It can also be used to screen MMP-3 inhibitors when a purified MMP-3 enzyme is used. With excellent fluorescence quantum yield and longer wavelength, the probe shows less interference from autofluorescence of test compounds and cellular components and is much more sensitive than an EDANS/Dabcyl FRET substrate. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/525 nm. The perfect excitation of the green probe at 488 nm makes the assay readily readable with almost all the common fluorescence instruments equipped with Argon laser and FITC filter set.

    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    ab112148 should be stored Desiccated

  • アプリケーション適用あり: Functional Studiesmore details

法規制情報

製品の特性

  • 機能Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 別名
    • CHDS6
    • Matrix metalloproteinase 3
    • Matrix metalloproteinase-3
    • MGC126102
    • MGC126103
    • MGC126104
    • MMP 3
    • MMP-3
    • MMP3
    • MMP3_HUMAN
    • Proteoglycanase
    • SL-1
    • SL1
    • STMY
    • STMY1
    • STR1
    • Stromelysin 1
    • Stromelysin-1
    • Transin 1
    • Transin-1
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab112148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

MMP-3 Activity Assay Kit (Fluorometric - Green) 画像

  • Dose response of MMP-3 enzyme activity.

    The APMA-activated MMP-3 was serially diluted in assay buffer, and then mixed with Green substrate. The fluorescence signal was monitored after one hour using a microplate reader at Ex/Em = 485/525 nm. The reading from all wells was subtracted with the reading from substrate control. As low as 1 ng/well of MMP-3 was detected (n=3). Note: MMP-3 from different sources varies in its endogenous activity.

プロトコール

MMP-3 Activity Assay Kit (Fluorometric - Green) (ab112148) 使用論文

ab112148 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Ab112148is not designed as specific in regards to species. The kit should recognize both species. Substrates can be digested by other MMPs too, but at slower rate than MMP3

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Thank you for your call today, and I apologize that the results with this kit were unsatisfactory. Your credit note ID is ***. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing departmen...

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Thank you again for your call last week and for forwarding your data on the substrate fluorescence. I have just heard back from the lab, and I'm told that it is normal for the green substrate to have residual fluorescence. This signal is typically 10-2...

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Thank you for faxing in the data. I have just received the document and am forwarding it to the lab for their feedback. I will get back to you as soon as possible, but please let me know if there is anything you need in the meantime. Enjoy your...

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Thank you for your reply and for sending the data. I am having trouble opening the data file. Would it be possible to re-send it as an Excel, Word, pdf, PowerPoint, or text file? I am hopeful that by reviewing the data, we can determine the source of t...

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Thank you for your call today and for letting us know about the problem with this kit. Please reply to this email and send any data that you can provide. I will be happy to forward this information to the lab to see if there are any suggestions they ca...

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Thank you for contacting us. The kit developer has informed me that if activated MMP3 is in the sample, then it will be capable of cleaving the peptide substrate, which creates the signal. So, in theory, APMA is not necessary. I recommend including a p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"