Anti-MMACHC/CblC 抗体 (ab96195)
Key features and details
- Rabbit polyclonal to MMACHC/CblC
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MMACHC/CblC antibody
MMACHC/CblC 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MMACHC/CblC -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to Human MMACHC/CblC aa 1-188.
Database link: NP_056321 -
ポジティブ・コントロール
- 293T and Raji whole cell lysates; HeLa cells; A431, H1299, HeLaS3 and HepG2 cell lines
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96195の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
May be involved in the binding and intracellular trafficking of cobalamin (vitamin B12). -
組織特異性
Widely expressed. Expressed at higher level in fetal liver. Also expressed in spleen, lymph node, thymus and bone marrow. Weakly or not expressed in peripheral blood leukocytes. -
パスウェイ
Cofactor biosynthesis; adenosylcobalamin biosynthesis. -
関連疾患
Defects in MMACHC are the cause of methylmalonic aciduria and homocystinuria type cblC (MMACHC) [MIM:277400]. MMACHC is a disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). Affected individuals may have developmental, hematologic, neurologic, metabolic, ophthalmologic, and dermatologic clinical findings. Although considered a disease of infancy or childhood, some individuals develop symptoms in adulthood. -
配列類似性
Belongs to the MMACHC family. - Information by UniProt
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参照データベース
- Entrez Gene: 513433 Cow
- Entrez Gene: 25974 Human
- Entrez Gene: 67096 Mouse
- Entrez Gene: 313520 Rat
- Omim: 609831 Human
- SwissProt: Q5E9C8 Cow
- SwissProt: Q9Y4U1 Human
- SwissProt: Q9CZD0 Mouse
see all -
別名
- 1810037K07Rik antibody
- BOS_3654 antibody
- cblC antibody
see all
画像
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All lanes : Anti-MMACHC/CblC antibody (ab96195) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 32 kDa
12% SDS-PAGE -
ab96195, at 1/100 dilution, staining MMACHC/CblC in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab96195 は 2 報の論文で使用されています。
- Fofou-Caillierez MB et al. Interaction between methionine synthase isoforms and MMACHC: characterization in cblG-variant, cblG and cblC inherited causes of megaloblastic anaemia. Hum Mol Genet N/A:N/A (2013). PubMed: 23825108
- Yu HC et al. An X-linked cobalamin disorder caused by mutations in transcriptional coregulator HCFC1. Am J Hum Genet 93:506-14 (2013). PubMed: 24011988