製品の概要

  • 製品名
    Anti-MLN64 antibody
  • 製品の詳細
    Rabbit polyclonal to MLN64
  • アプリケーション
    適用あり: ICC/IF, IHC-P, ICC, IP, Inhibition Assay, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Hamster, Cow, Human
  • 免疫原

    Other Immunogen Type corresponding to MLN64. Recombinant MLN64 protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. By Western blot, this antibody detects an ~53 kDa protein representing MLN 64 from rat adrenal gland tissue extracts. This antibody also detects an ~25 kDa protein from rat adrenal gland which may correspond to degradation product.

ターゲット情報

  • 機能
    Binds and transports cholesterol. Promotes steroidogenesis in placenta and brain.
  • 配列類似性
    Contains 1 MENTAL domain.
    Contains 1 START domain.
  • 細胞内局在
    Late endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAB 1 antibody
    • CAB1 antibody
    • Es 64 antibody
    • Es64 antibody
    • FLJ41370 antibody
    • Metastatic lymph node gene 64 protein antibody
    • Metastatic lymph node protein 64 antibody
    • MLN 64 antibody
    • Mln64 antibody
    • Protein CAB 1 antibody
    • Protein CAB1 antibody
    • Protein MLN 64 antibody
    • Protein MLN64 antibody
    • StAR related lipid transfer protein 3 antibody
    • StAR-related lipid transfer protein 3 antibody
    • STAR3_HUMAN antibody
    • STARD 3 antibody
    • StARD3 antibody
    • START domain containing 3 antibody
    • START domain-containing protein 3 antibody
    • Steroidogenic acute regulatory protein related antibody
    see all

画像

  • Anti-MLN64 antibody (ab3478) at 1 µg/ml + Lysates prepared from rat adrenal gland

    Predicted band size : 49 kDa
    Western blot of MLN64 on rat adrenal gland extract using ab3478.
  • ab3478 staining MLN64 in Human ovarian cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours 10 minutes at 4°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 20 minutes at 25°C. An undiluted HRP-conjugated monoclonal was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab3478 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Balboa E  et al. MLN64 induces mitochondrial dysfunction associated with increased mitochondrial cholesterol content. Redox Biol 12:274-284 (2017). WB . Read more (PubMed: 28282615) »
  • Qiu Y  et al. Association analysis of ERBB2 amplicon genetic polymorphisms and STARD3 expression with risk of gastric cancer in the Chinese population. Gene 535:225-32 (2014). IHC-P ; Human . Read more (PubMed: 24291029) »

See all 9 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ovarian cancer)
Specification
Ovarian cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
BSA as blocking agent for 12 hour(s) and 10 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mrs. Wu Xin

Verified customer

投稿 Oct 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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