製品の概要

  • 製品名
    Anti-MLK4 antibody - N-terminal
    MLK4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MLK4 - N-terminal
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide from the N-terminal region of human MLK4 conjugated to KLH.

  • ポジティブ・コントロール
    • 293 cell lysates transiently transfected with the MLK4 gene. Human colon carcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    ab71510 is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab71510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Predicted molecular weight: 114 kDa.

ターゲット情報

  • 機能
    Activates the JUN N-terminal pathway.
  • 配列類似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • 翻訳後修飾
    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ862P8.3 antibody
    • KIAA1804 antibody
    • M3KL4_HUMAN antibody
    • Mitogen activated protein kinase kinase kinase antibody
    • Mitogen-activated protein kinase kinase kinase MLK4 antibody
    • Mixed lineage kinase 4 antibody
    • MLK 4 antibody
    • Mlk4 antibody
    • RP5 862P8.2 antibody
    see all

画像

  • All lanes : Anti-MLK4 antibody - N-terminal (ab71510) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the MLK4 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 114 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 130 kDa,50 kDa,72 kDa. We are unsure as to the identity of these extra bands.
  • ab71510 at 1/10 dilution, staining MLK4 in human colon carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

参考文献

ab71510 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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