Rabbit polyclonal to MLK4 - N-terminal
Synthetic peptide from the N-terminal region of human MLK4 conjugated to KLH.
293 cell lysates transiently transfected with the MLK4 gene. Human colon carcinoma tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein A purified
ab71510 is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/50 - 1/100. Predicted molecular weight: 114 kDa.
Activates the JUN N-terminal pathway.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. Contains 1 SH3 domain.
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
Information by UniProt
Western blot - MLK4 antibody - N-terminal (ab71510)
All lanes :
Anti-MLK4 antibody - N-terminal (ab71510) at 1/50 dilution
Lane 1 :
Nontransfected 293 cell lysate
Lane 2 :
293 cell lysate transiently transfected with the MLK4 gene
Lysates/proteins at 2 µg per lane.
Predicted band size:
Observed band size:
105 kDa (
why is the actual band size different from the predicted?
Additional bands at:
130 kDa, 50 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MLK4 antibody - N-terminal (ab71510)
ab71510 at 1/10 dilution, staining MLK4 in human colon carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
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