製品の概要

  • 製品名Anti-MLK4 antibody
    MLK4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MLK4
  • アプリケーション適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 986-1036 of Human MLK4 (NP_115811.2)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa and 293T cells This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab93798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Activates the JUN N-terminal pathway.
  • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • 翻訳後修飾Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ862P8.3 antibody
    • KIAA1804 antibody
    • M3KL4_HUMAN antibody
    • Mitogen activated protein kinase kinase kinase antibody
    • Mitogen-activated protein kinase kinase kinase MLK4 antibody
    • Mixed lineage kinase 4 antibody
    • MLK 4 antibody
    • Mlk4 antibody
    • RP5 862P8.2 antibody
    see all

Anti-MLK4 antibody 画像

  • ICC/IF image of ab93798 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93798 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-MLK4 antibody (ab93798) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 114 kDa


    Exposure time : 10 seconds
  • Detection of MLK4 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab93798 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.

Anti-MLK4 antibody (ab93798) 使用論文

ab93798 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"