Anti-MLK4 抗体 (ab93798)
Key features and details
- Rabbit polyclonal to MLK4
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MLK4 antibody -
製品の詳細
Rabbit polyclonal to MLK4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 986-1036 of Human MLK4 (NP_115811.2)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93798の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 114 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Activates the JUN N-terminal pathway. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain. -
翻訳後修飾
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. - Information by UniProt
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参照データベース
- Entrez Gene: 84451 Human
- SwissProt: Q5TCX8 Human
- Unigene: 547779 Human
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別名
- dJ862P8.3 antibody
- KIAA1804 antibody
- M3KL4_HUMAN antibody
see all
画像
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All lanes : Anti-MLK4 antibody (ab93798) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Exposure time: 10 seconds -
Detection of MLK4 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab93798 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab93798 は 1 報の論文で使用されています。
- Xi Y et al. Mixed lineage kinase-4 promotes gastric carcinoma tumorigenesis through suppression of the c-Jun N-terminal kinase signaling pathway. Exp Ther Med 16:3317-3324 (2018). PubMed: 30233678