Anti-MLF1 Interacting Protein/PBIP1 (phospho T78) 抗体 (ab115570)
Key features and details
- Rabbit polyclonal to MLF1 Interacting Protein/PBIP1 (phospho T78)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-MLF1 Interacting Protein/PBIP1 (phospho T78) antibody -
製品の詳細
Rabbit polyclonal to MLF1 Interacting Protein/PBIP1 (phospho T78) -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Chimpanzee -
免疫原
Synthetic phosphopeptide conjugated to KLH, corresponding to amino acids surrounding Thr78 of Human MLF1 Interacting Protein/PBIP1.
-
ポジティブ・コントロール
- Human prostate tissue
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115570の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
---|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression. -
組織特異性
Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines. -
翻訳後修飾
Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1. -
細胞内局在
Cytoplasm. Nucleus. Chromosome > centromere > kinetochore. Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates. - Information by UniProt
-
参照データベース
- Entrez Gene: 617866 Cow
- Entrez Gene: 79682 Human
- Entrez Gene: 71876 Mouse
- Entrez Gene: 306464 Rat
- Omim: 611511 Human
- SwissProt: Q2KIW5 Cow
- SwissProt: Q71F23 Human
- SwissProt: Q8C4M7 Mouse
see all -
別名
- CENP U antibody
- CENP-50 antibody
- CENP-U antibody
see all
画像
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab115570 は論文での使用が確認できていません。