製品の概要

  • 製品名Anti-MKRN2 antibody
    MKRN2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MKRN2
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 366 and the C-terminus (residue 416) of human MKRN2 (NP_054879.3).

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 47 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

Anti-MKRN2 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MKRN2 with ab72055 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MKRN2 antibody (ab72055) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg


    Predicted band size : 47 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human MKRN2 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72055 at 3 µg/mg lysate (Lane 1). Lane 2 represents Control IgG IP. Subsequent Western blot detection was done using ab72055 at 1 µg/ml.

Anti-MKRN2 antibody (ab72055) 使用論文

ab72055 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"