製品の概要

  • 製品名Anti-MKRN1 antibody
    MKRN1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MKRN1
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 432 and the C-terminus (residue 482) of human MKRN1 (NP_038474.1).

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa and 293T cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
  • 組織特異性Ubiquitous.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 4 C3H1-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • 翻訳後修飾Auto-ubiquitinated; which leads to proteasomal degradation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase makorin-1 antibody
    • FLJ21334 antibody
    • HGNC:7112 antibody
    • Makorin antibody
    • Makorin ring finger protein 1 antibody
    • MKRN 1 antibody
    • mkrn1 antibody
    • MKRN1_HUMAN antibody
    • OTTHUMP00000212117 antibody
    • OTTHUMP00000212118 antibody
    • OTTHUMP00000212121 antibody
    • OTTHUMP00000212124 antibody
    • Ring finger protein 1 antibody
    • RING finger protein 61 antibody
    • RNF61 antibody
    see all

Anti-MKRN1 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MKRN1 with ab72054 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MKRN1 antibody (ab72054) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 100 kDa,16 kDa,28 kDa,45 kDa. We are unsure as to the identity of these extra bands.

Anti-MKRN1 antibody (ab72054) 使用論文

This product has been referenced in:
  • Panneer Selvam S  et al. Binding of the sphingolipid S1P to hTERT stabilizes telomerase at the nuclear periphery by allosterically mimicking protein phosphorylation. Sci Signal 8:ra58 (2015). Read more (PubMed: 26082434) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"