製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MKRN1
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 432 and the C-terminus (residue 482) of human MKRN1 (NP_038474.1).

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa and 293T cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
  • 組織特異性
    Ubiquitous.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 4 C3H1-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • 翻訳後修飾
    Auto-ubiquitinated; which leads to proteasomal degradation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase makorin-1 antibody
    • FLJ21334 antibody
    • HGNC:7112 antibody
    • Makorin antibody
    • Makorin ring finger protein 1 antibody
    • MKRN 1 antibody
    • mkrn1 antibody
    • MKRN1_HUMAN antibody
    • OTTHUMP00000212117 antibody
    • OTTHUMP00000212118 antibody
    • OTTHUMP00000212121 antibody
    • OTTHUMP00000212124 antibody
    • Ring finger protein 1 antibody
    • RING finger protein 61 antibody
    • RNF61 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MKRN1 with ab72054 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MKRN1 antibody (ab72054) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 100 kDa,16 kDa,28 kDa,45 kDa. We are unsure as to the identity of these extra bands.

参考文献

This product has been referenced in:
  • Panneer Selvam S  et al. Binding of the sphingolipid S1P to hTERT stabilizes telomerase at the nuclear periphery by allosterically mimicking protein phosphorylation. Sci Signal 8:ra58 (2015). WB ; Human . Read more (PubMed: 26082434) »
  • Cassar PA  et al. Integrative genomics positions MKRN1 as a novel ribonucleoprotein within the embryonic stem cell gene regulatory network. EMBO Rep 16:1334-57 (2015). WB, Flow Cyt . Read more (PubMed: 26265008) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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