The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
Use at an assay dependent concentration. PubMed: 23243284
Use a concentration of 1.25 µg/ml. Detects a band of approximately 109 kDa (predicted molecular weight: 99 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:1562500.
Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation.
Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain.
Note=A chromosomal aberration involving MKL1 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with RBM15. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.
Contains 2 RPEL repeats. Contains 1 SAP domain.
The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity. The RPEL repeats mediate binding to globular actin.
Cytoplasm. Nucleus. Binding to globular actin is required to maintain cytoplasmic localization.
ICC/IF image of ab49311 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49311, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-Mkl1 antibody (ab49311)This image is courtesy of an anonymous Abreview.
Paraformaldehyde-fixed, 0.3% Triton X100 permeabilized HeLa (humanepithelial cell line from cervix adenocarcinoma) cells stained for Mkl1 (red) using ab49311 at 1/150 dilution in ICC/IF, followed by Goat anti Rabbit Cy3 secondary antibody at 1/100 dilution.