製品の概要

  • 製品名Anti-Mixl1 antibody
    Mixl1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Mixl1
  • アプリケーション適用あり: WBmore details
  • 種交差性

    交差が予測される動物種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Mouse Mixl1.

    (Peptide available as ab30617.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Murine ES WCL expressing mesoderm Marker lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

ターゲット情報

  • 機能Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression.
  • 組織特異性Restricted to progenitors and secondary lymph tissues. In normal hematopoiesis, it is restricted to immature B-and T-lymphoid cells. Present in differentiating embryonic stem cells (at protein level).
  • 配列類似性Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 翻訳後修飾Phosphorylated at multiple sites.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hMix antibody
    • Homeobox protein MIXL1 antibody
    • Homeodomain protein 1 antibody
    • Homeodomain protein MIX antibody
    • MILD1 antibody
    • MIX antibody
    • Mix.1 antibody
    • Mix.1 homeobox-like protein antibody
    • Mix1 homeobox-like 1 antibody
    • MIX1 homeobox-like protein 1 antibody
    • MIXL antibody
    • Mixl1 antibody
    • MIXL1_HUMAN antibody
    see all

Anti-Mixl1 antibody 画像

  • Anti-Mixl1 antibody (ab28411) at 1 µg/ml + Whole Cell Lysate from Murine ES Cells Differentiated to Express Mesoderm Markers at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.

Anti-Mixl1 antibody (ab28411) 使用論文

ab28411 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Day 4 differentiated Mouse Embryonic Stem Cells)
Specification Day 4 differentiated Mouse Embryonic Stem Cells
Fixative Formaldehyde
Permeabilization Yes - PBS-0.3% Tween
Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Dr. Vladimir Mazurov

Verified customer

投稿 Aug 25 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E6.5 embryo)
Specification E6.5 embryo
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization Yes - Tween
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Ms. Mary Trask

Verified customer

投稿 Jan 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"