MitoTox™ Complex IV OXPHOS Activity Microplate Assay (ab109906)

製品の概要

  • 製品名MitoTox™ Complex IV OXPHOS Activity Microplate Assay
    Complex IV キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 test
  • アッセイタイプDirect
  • 全工程の試験時間
    5h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Cow, Human
  • 製品の概要

    ab109906 MitoTox™ OXPHOS Complex IV Activity Kit (MTOX4) is designed for screening the direct inhibitory effect of compounds on Complex IV activity and does this using enzyme immunocaptured from Bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex IV.

  • 特記事項

    Store at -80°C: Bovine heart mitochondria

    Store at -20°C: Reagent C (store at -80°C for long term storage)

    Store at 4°C: Pre-coated 96-well plate, Blocking Buffer, Mito Buffer, Detergent

    Store at room temperature or 4°C: Blocking powder and reagent reservoirs

  • アプリケーション適用あり: Functional Studiesmore details
  • 試験プラットフォームMicroplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

MitoTox™ Complex IV OXPHOS Activity Microplate Assay 画像

プロトコール

MitoTox™ Complex IV OXPHOS Activity Microplate Assay (ab109906) 使用論文

This product has been referenced in:
  • Chin RM  et al. The metabolite a-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR. Nature 509:397-401 (2014). Read more (PubMed: 24828042) »
  • Lai K  et al. Integrated Compound Profiling Screens Identify the Mitochondrial Electron Transport Chain as the Molecular Target of the Natural Products Manassantin, Sesquicillin, and Arctigenin. ACS Chem Biol N/A:N/A (2012). Read more (PubMed: 23138533) »

See all 2 Publications for this product

Product Wall

The kits that are the best suited for your experiment are listed below. I am having our datasheets team update the species reactivity.
ab109903, Complex I; reactivity = Human, mouse, rat, bovine
http://www.abcam.com/mitotox%E2%84%A2-complex-i...

Read More

This is because the plate requires fresh blocking to prevent non specific binding of the enzyme to the plate. We believe that this occurs because CIV is a highly hydrophobic enzyme.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"