MitoTox™ Complex II OXPHOS Activity Microplate Assay (ab109904)

製品の概要

  • 製品名MitoTox™ Complex II OXPHOS Activity Microplate Assay
    Complex II キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • アッセイタイプDirect
  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse, Cow, Human
  • 製品の概要

    MitoTox™ Complex II OXPHOS Activity Microplate Assay is designed for testing the direct inhibitory effect of compounds on Complex II activity using enzyme immunocaptured from Bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex II.

  • 特記事項

    Please store Succinate, Ubiquinone 2, BHM, DCPIP at -80°C and all other components at 4°C.

  • アプリケーション適用あり: Functional Studiesmore details
  • 試験プラットフォームMicroplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

MitoTox™ Complex II OXPHOS Activity Microplate Assay 画像

プロトコール

MitoTox™ Complex II OXPHOS Activity Microplate Assay (ab109904) 使用論文

This product has been referenced in:
  • Landry GM  et al. Diglycolic acid inhibits succinate dehydrogenase activity in human proximal tubule cells leading to mitochondrial dysfunction and cell death. Toxicol Lett 221:176-84 (2013). Read more (PubMed: 23827505) »
  • Lai K  et al. Integrated Compound Profiling Screens Identify the Mitochondrial Electron Transport Chain as the Molecular Target of the Natural Products Manassantin, Sesquicillin, and Arctigenin. ACS Chem Biol N/A:N/A (2012). Read more (PubMed: 23138533) »

See all 2 Publications for this product

Product Wall

The kits that are the best suited for your experiment are listed below. I am having our datasheets team update the species reactivity.
ab109903, Complex I; reactivity = Human, mouse, rat, bovine
http://www.abcam.com/mitotox%E2%84%A2-complex-i...

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ab109904 measures solely complex II, whereas ab109905 measures complexes II+III.
We do not offer an immunocapture activity assay for Complex III only. The reason is that we have not generated an antibody capable of immunocapturing the native enz...

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Thank you for contacting us.

The 10x detergent in the kit is 10% Lauryl maltoside, which can also be purchased separately as ab109857.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any...

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Thank you for your call and for your patience while I've been in touch with the lab about ab109904.

My contact at the lab gave the following reply: "Yes, a pH of 2 will destroy the assay. You will need to bring the pH to neutral ( 7 &nda...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"