MitoTox™ Complex I OXPHOS Activity Microplate Assay (ab109903)


  • 製品名MitoTox™ Complex I OXPHOS Activity Microplate Assay
    Complex I キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • アッセイタイプQuantitative
  • 全工程の試験時間
    5h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 製品の概要

    MitoTox™ Complex I OXPHOS Activity Microplate Assay (ab109903) is designed for screening the direct inhibitory effect of compounds on Complex I activity and does this using enzyme immunocaptured from bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex I. The phospholipids provided in the kit are essential for rotenone-sensitive Complex I activity, rotenone being a well-known inhibitor of Complex I.

  • 特記事項

    Store Microplate, Wash buffer, Mito Buffer, and detergent at 4°C. Reagent reservoirs may be stored at RT. Store Phospholipids at -20°C. Store Bovine heart mitochondria, Ubiquinone and Activity Buffer at -80°C.

  • アプリケーション適用あり: Functional Studiesmore details
  • 試験プラットフォームMicroplate


  • 別名
    • NADH dehydrogenase


Our Abpromise guarantee covers the use of ab109903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

MitoTox™ Complex I OXPHOS Activity Microplate Assay 画像

  • Dose Response Assay using Rotenone


MitoTox™ Complex I OXPHOS Activity Microplate Assay (ab109903) 使用論文

This product has been referenced in:
  • Kalghatgi S  et al. Bactericidal antibiotics induce mitochondrial dysfunction and oxidative damage in Mammalian cells. Sci Transl Med 5:192ra85 (2013). Read more (PubMed: 23825301) »
  • Lai K  et al. Integrated Compound Profiling Screens Identify the Mitochondrial Electron Transport Chain as the Molecular Target of the Natural Products Manassantin, Sesquicillin, and Arctigenin. ACS Chem Biol N/A:N/A (2012). Read more (PubMed: 23138533) »

See all 2 Publications for this product

Product Wall

The plate is one solid plate and not removable strips. Plate is best used at once as there is risk to unused wells getting wet, etc. We have not tried this and therefore cannot guarantee, but you could try putting a plate seal over...

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The kits that are the best suited for your experiment are listed below. I am having our datasheets team update the species reactivity.
ab109903, Complex I; reactivity = Human, mouse, rat, bovine

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Thank you for contacting us.

I have checked with our MitoSciences lab and they sent the attached QC data for the plates used in that lot. Additionally, these kits are designed to be run with developmental compounds. On occasion, we have seen ...

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Les phospholipides peuvent en effet être le paramètre critique pour le bon fonctionnement du kit MTOX I.Ils ne sont pas stables si ils sont conservés à 4ºC et doivent être impérativement stockés &agra...

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Je suis ravi que les suggestions aient été utiles.

J'ai transmis votre question au laboratoire et vous contacterai de nouveau dès que j'aurai reçu une réponse.

Merci de votre réponse.

Le laboratoire vient de m'indiquer que la valeur de laDO initiale mesurée lors des tests de contrôle qualité était de 1 pour le lot GR78475-2.

Le problème rencontr&eac...

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Merci de nous avoir contactés.

La DO initiale (0.5) est en effet un peu faible et pourrait expliquer la valeur anormalementélevée de l'IC50.

Une des raisons expliquant la faible DO est l'age du kit et les condit...

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Thank you for your enquiry.

The solvent for the Ubiquinone 1 component is 100% ethanol.

Thank you for your enquiry. Please find the answers to your questions below.

1) Thank you for your feedback. We will take this into consideration with the production team.

2) Typically customers have used this kit in combination w...

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Thank you for your enquiry and your interest in our products. ab109905 is the only assay which is not an immunoassay. The supplied bovine sample can be replaced with a mitochondrial sample from any other species. This may require some optimization ...

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