MitoTox™ Complete OXPHOS Activity Assay Kit (5 Assays) (ab110419)

製品の概要

  • 製品名MitoTox™ Complete OXPHOS Activity Assay Kit (5 Assays)
  • 検出方法Colorimetric
  • アッセイタイプQuantitative
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Cow, Human
  • 製品の概要

    The assays measure the direct effects of drugs on the 5 OXPHOS complexes. Complexes I,II, IV and V are each immunocaptured from bovine heart mitochondria (included) in 96-well plates, and their activities are measured by simple spectrophotometric assays. Complex III is measured in an in-solution colorimetric assay.

  • アプリケーション適用あり: Functional Studiesmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 保存方法Please refer to protocols.
  • 内容 5 x 96 tests
    10X Detergent 1 x 1ml
    12-channel reagent reservoirs 10 units
    1X Blocking Buffer (Solution 1) 1 x 70ml
    1X Complex III Mito Buffer 1 x 1ml
    1X Complex IV Mito Buffer (Solution 2) 1 x 110ml
    1X Mito Buffer 2 x 5ml
    1X Succinate Solution 1 x 12ml
    20 x Buffer 1 x 5ml
    20X Wash Buffer 2 x 5ml
    96-well microplate 1 unit
    Bovine heart mitochondria 4 x 360µl
    Bovine Heart Mitochondria 1 x 90µl
    Bovine heart mitochondria (5 mg/mL) 1 x 300µl
    Complex I Activity Buffer 1 x 24ml
    Complex II Activity Buffer 1 x 24ml
    Complex V Activity Buffer 1 x 24ml
    Cytochrome c (oxidized) 1 x 550µl
    DCPIP 1 x 250µl
    Detergent 1 x 20µl
    Detergent 2 x 100µl
    Detergent (ab109906) 1 x 20µl
    Fat free powdered milk (Blocking powder) 1 x 1.8g
    Phospholipid 2 x 6ml
    Pre-coated 96-well microplate 1 unit
    Pre-coated 96-well microplate 1 unit
    Pre-coated 96-well microplate 1 unit
    Pre-coated 96-well microplate 1 unit
    Reagent C (Cytochrome c) 2 x 1ml
    Single-channel reagent reservoirs 2 units
    Succinate 1 x 500µl
    Ubiquinone 1 1 x 60µl
    Ubiquinone 2 1 x 60µl
  • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab110419 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent dilution.

    MitoTox™ Complete OXPHOS Activity Assay Kit (5 Assays) 画像

    プロトコール

    MitoTox™ Complete OXPHOS Activity Assay Kit (5 Assays) (ab110419) 使用論文

    This product has been referenced in:
    • Lai K  et al. Integrated Compound Profiling Screens Identify the Mitochondrial Electron Transport Chain as the Molecular Target of the Natural Products Manassantin, Sesquicillin, and Arctigenin. ACS Chem Biol N/A:N/A (2012). Read more (PubMed: 23138533) »

    See 1 Publication for this product

    Product Wall

    On our datasheet we state that this kit can be used with human and cow.

    Therefore yes I can confirm that you can use it on your human samples. What I think may be confusing is that we supply bovine heart mitochondria rather than human.
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    It is crucial that this type of data is analyzed based on the actual kinetic trace (via softmax software). When a reduction trace is superimposed you will notice that the kinetic change in signal is not constant for the entire assay. The speed of ...

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    The mitotox kits will work with mitochondria from any human tissue. Therefore it will work with mitochondria from human PBMCs.


    To isolate mitochondria from human PBMCs I suggest the customer to use ab110170 (Mitochondria Isolation Kit f...

    Read More

    The kits that are the best suited for your experiment are listed below. I am having our datasheets team update the species reactivity.
    ab109903, Complex I; reactivity = Human, mouse, rat, bovine
    http://www.abcam.com/mitotox%E2%84%A2-complex-i...

    Read More

    Thank you for contacting Abcam.

    MitoSciences is part of the Abcam family now, and I have directly contacted them regarding your inquiry. They responded that the 384 well plate format has not been validated or produced. Since Abcam and MitoScie...

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    Thank you for contacting Abcam.

    I contacted the supplier, and they responded that ab110419 has not been validated in 384 well plate format.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need an...

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    Thank you for contacting Abcam.

    We only make custom products for bulk orders of 10 or more products. If you would like to inquire about placing a bulk order for ab110419 in 384-well format please reply to this email, and we will see if the su...

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    Thank you for contacting us.
    The assays measure the direct effects of drugs on the 5 OXPHOS complexes and can be used on human samples.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more...

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    I now have further information to share with you.

    The antibodies used in the MitoTox™ Complete OXPHOS Activity Assay Kit (5 Assays), ab110419,to coat the plates are cross-reactive againstthe human proteins. The kit can therefore be used...

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    Thank you for contacting us and your interest in our products.

    The MitoTox™ Complete OXPHOS Activity Assay Kit (5 Assays), ab110419, provides bovine heart mitochondria to allow for complexes I, II, IV and V to be immunecaptured and thei...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"