Anti-Mitotic proteins 抗体 [0.T.181] (ab14581)


  • 製品名Anti-Mitotic proteins antibody [0.T.181]
  • 製品の詳細
    Mouse monoclonal [0.T.181] to Mitotic proteins
  • 特異性Recognizes a phosphorylated epitope (S/T)P found in phosphoproteins such as MAP2, HSP70, cdc25 and DNA topoisomerase IIa, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM-2 varies from species to species and with the cell type.
  • アプリケーション適用あり: IHC-P, ICC/IF, IP, WB, ICC, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: a wide range of other species
  • 免疫原

    Tissue/ cell preparation (Mitotic human HeLa cell cytosolic lysate).

  • ポジティブ・コントロール
    • Colcemid treated HeLa cell lysate.
  • 特記事項For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.



Our Abpromise guarantee covers the use of ab14581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 µg/ml.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 92 kDa.
ICC Use a concentration of 5 - 15 µg/ml.
ELISA Use at an assay dependent concentration. This antibody was used in a non-radioactive, dissociation-enhanced time resolved fluoroimmunoassa.Active enzymes were used to phosphorylate substrate peptides. The phosphorylated peptides were detected using MPM2 in conjunction with a europium (Eu3+) labeled secondary antibody. The phosphorylated peptides detected include: S1000-50 Serine Kinase Substrate Peptide (Biotin) (RRRAPLSP-N-Biotin) and S1000-80 Serine/Threonine Kinase Substrate Peptide, Recombinant, Human (Biotin).


  • 細胞内局在The phosphoproteins have been found in the cytoplasm of mitotic cells and near centrosomes, kinetochores, and midbodies of the microtubule organizing centers.

Anti-Mitotic proteins antibody [0.T.181] 画像

  • Ab14581 staining human normal skin. Staining is localised to the nucleus.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab14581 staining Mitotic proteins - Mitosis Marker in Human Saos-2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500) for 1 hour. An Alexa Fluor® 594-conjugated Goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

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Anti-Mitotic proteins antibody [0.T.181] (ab14581) 使用論文

This product has been referenced in:
  • Machicoane M  et al. SLK-dependent activation of ERMs controls LGN-NuMA localization and spindle orientation. J Cell Biol 205:791-9 (2014). Read more (PubMed: 24958772) »
  • Arai A  et al. RECQL1 and WRN Proteins Are Potential Therapeutic Targets in Head and Neck Squamous Cell Carcinoma. Cancer Res 71:4598-607 (2011). Read more (PubMed: 21571861) »

See all 7 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (saos-2 human osteosarcoma)
Specification saos-2 human osteosarcoma
Fixative Paraformaldehyde
Permeabilization Yes - pbs triton 0.25%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C

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投稿 Apr 19 2012

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The information sent is not restricted to lot427148 only, it refers to all lots.I sincerely apologize about the mistake made - which has been corrected in the meantime.

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