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Tissue/ cell preparation (Mitotic human HeLa cell cytosolic lysate).
Our Abpromise guarantee covers the use of ab14581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 92 kDa.|
|ICC||Use a concentration of 5 - 15 µg/ml.|
|ELISA||Use at an assay dependent concentration. This antibody was used in a non-radioactive, dissociation-enhanced time resolved fluoroimmunoassa.Active enzymes were used to phosphorylate substrate peptides. The phosphorylated peptides were detected using MPM2 in conjunction with a europium (Eu3+) labeled secondary antibody. The phosphorylated peptides detected include: S1000-50 Serine Kinase Substrate Peptide (Biotin) (RRRAPLSP-N-Biotin) and S1000-80 Serine/Threonine Kinase Substrate Peptide, Recombinant, Human (Biotin).|