Anti-Mitofusin 2 抗体 (ab50843)
Key features and details
- Rabbit polyclonal to Mitofusin 2
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Mitofusin 2 antibody
Mitofusin 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Mitofusin 2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Cynomolgus monkey, Orangutan -
免疫原
Synthetic peptide corresponding to Human Mitofusin 2 aa 557-576 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:C-LGPKNSRRALMGYNDQVQRP
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50843の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 84 kDa).
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特記事項 |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 84 kDa). |
ターゲット情報
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機能
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions. -
組織特異性
Ubiquitous; expressed at low level. Highly expressed in heart and kidney. -
関連疾患
Charcot-Marie-Tooth disease 2A2
Neuropathy, hereditary motor and sensory, 6A -
配列類似性
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
Contains 1 dynamin-type G (guanine nucleotide-binding) domain. -
翻訳後修飾
Phosphorylated by PINK1.
Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion. -
細胞内局在
Mitochondrion outer membrane. Colocalizes with BAX during apoptosis. - Information by UniProt
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参照データベース
- Entrez Gene: 170731 Mouse
- Entrez Gene: 64476 Rat
- SwissProt: Q80U63 Mouse
- SwissProt: Q8R500 Rat
- Unigene: 154312 Mouse
- Unigene: 437499 Mouse
- Unigene: 8570 Rat
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別名
- CMT2A antibody
- CMT2A2 antibody
- CPRP 1 antibody
see all
画像
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All lanes : Anti-Mitofusin 2 antibody (ab50843) at 0.25 µg/ml
Lane 1 : Rat brain mitochondria
Lane 2 : Mouse brain mitochondria
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminescent substrate.
Predicted band size: 84 kDa
Observed band size: 86 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab50843 は 14 報の論文で使用されています。
- Delmotte P et al. TNFα induces mitochondrial fragmentation and biogenesis in human airway smooth muscle. Am J Physiol Lung Cell Mol Physiol 320:L137-L151 (2021). PubMed: 33146568
- Park S et al. Long-term depletion of cereblon induces mitochondrial dysfunction in cancer cells. BMB Rep 54:305-310 (2021). PubMed: 33408001
- Watanabe D et al. Predominant cause of faster force recovery in females than males after intense eccentric contractions in mouse fast-twitch muscle. J Physiol 599:4337-4356 (2021). PubMed: 34368970
- Yanagi T et al. Loss of dynamin-related protein 1 (Drp1) does not affect epidermal development or UVB-induced apoptosis but does accelerate UVB-induced carcinogenesis. J Dermatol Sci 99:109-118 (2020). PubMed: 32636049
- He M et al. Deletion of Mitochondrial Uncoupling Protein 2 Exacerbates Mitochondrial Damage in Mice Subjected to Cerebral Ischemia and Reperfusion Injury under both Normo- and Hyperglycemic Conditions. Int J Biol Sci 16:2788-2802 (2020). PubMed: 33061796