製品の概要

  • 製品名Anti-Mitofusin 2 antibody
    Mitofusin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Mitofusin 2
  • アプリケーション適用あり: WB, IP, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Duck, Pygoscelis adeliae (Penguin)
    交差が予測される動物種: Cynomolgus Monkey, Orangutan
  • 免疫原

    Synthetic peptide:

    KINGIFEQLGAYIQESAT

    with C-terminal added cysteine, conjugated to KLH, corresponding to N terminal amino acids 38-55 of Human Mitofusin 2

  • ポジティブ・コントロール
    • Rat brain mitochondrial lysate; mouse brain mitochondrial lysate; differentiated mouse C2 cells; HeLa cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
IP Use at an assay dependent concentration.

5-10µg

ICC/IF Use a concentration of 20 - 30 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
  • 組織特異性Ubiquitous; expressed at low level. Highly expressed in heart and kidney.
  • 関連疾患Charcot-Marie-Tooth disease 2A2
    Neuropathy, hereditary motor and sensory, 6A
  • 配列類似性Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
    Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
  • 翻訳後修飾Phosphorylated by PINK1.
    Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.
  • 細胞内局在Mitochondrion outer membrane. Colocalizes with BAX during apoptosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CMT2A antibody
    • CMT2A2 antibody
    • CPRP 1 antibody
    • CPRP1 antibody
    • EC 3.6.5.- antibody
    • Fzo antibody
    • HSG antibody
    • hyperplasia suppressor gene antibody
    • Hypertension related protein 1 antibody
    • KIAA0214 antibody
    • MARF antibody
    • MFN 2 antibody
    • Mfn2 antibody
    • MFN2_HUMAN antibody
    • Mitochondrial assembly regulatory factor antibody
    • Mitofusin-2 antibody
    • Mitofusin2 antibody
    • Transmembrane GTPase MFN2 antibody
    see all

Anti-Mitofusin 2 antibody 画像

  • Lane 1 : Anti-Mitofusin 2 antibody (ab50838) at 0.5 µg/ml
    Lanes 2 - 3 : Anti-Mitofusin 2 antibody (ab50838) at 1 µg/ml
    Lane 4 : Anti-Mitofusin 2 antibody (ab50838) at 2 µg/ml

    Lane 1 : rat brain mitochondria extract
    Lane 2 : rat brain mitochondria extract
    Lane 3 : rat brain mitochondria extract with Mitofusin 2 immunizing peptide at 5 µg/ml
    Lane 4 : rat brain mitochondria extract

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminescent substrate
    Developed using the ECL technique

    Predicted band size : 86 kDa
    Observed band size : 86 kDa

Anti-Mitofusin 2 antibody (ab50838) 使用論文

This product has been referenced in:
  • Kühl I  et al. POLRMT regulates the switch between replication primer formation and gene expression of mammalian mtDNA. Sci Adv 2:e1600963 (2016). WB . Read more (PubMed: 27532055) »
  • Feng H  et al. Training-induced mitochondrial adaptation: role of peroxisome proliferator-activated receptor ? coactivator-1a, nuclear factor-?B and ß-blockade. Exp Physiol 98:784-95 (2013). WB ; Rat . Read more (PubMed: 23104933) »

See all 9 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (h9c2)
Specification h9c2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Mar 18 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Chinese Hamster Cell lysate - whole cell (CHO cell)
Specification CHO cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Feb 10 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (LIVER)
Specification LIVER
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

投稿 Jul 29 2014

Thank you for contacting Abcam regarding ab50838.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check th...

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Application Western blot
Sample Cairina moschata Cell lysate - other (isolated muscle mitochondria)
Loading amount 50 µg
Specification isolated muscle mitochondria
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Mar 08 2012

Thank you for contacting us.

The rabbit polyclonal I recommended for IHC with mouse tissue is ab16056, the COX IV antibody. If you already have a COX IV antibody, I recommend trying that first. Antibody ab16056 is not guaranteed for IHC on f...

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Merci de nous avoir contactés. Comme convenu, je vous transfère le numéro de commande de remplacement gratuit du ab50838  : ******. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition. En échange, nous serions ...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human keratinocytes)
Specification human keratinocytes
Fixative Paraformaldehyde
Permeabilization Yes - 0,25 triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

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Verified customer

投稿 May 28 2011

Application Western blot
Sample Human Cell lysate - whole cell (human immortalized keratinocytes and cervical tumo)
Loading amount 50 µg
Specification human immortalized keratinocytes and cervical tumo
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (Isolated brain mitochondria)
Loading amount 20 µg
Specification Isolated brain mitochondria
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Jia Yao

Verified customer

投稿 Sep 17 2009

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"