製品の概要

  • 製品名Anti-Mitofusin 1 antibody
    Mitofusin 1 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to Mitofusin 1
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human Mitofusin 1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HeLa whole cell lysate and in the following tissue lysates: Human Kidney; Mouse Kidney; Rat Kidney; Human Heart; Mouse Heart; Rat Heart. This antibody gave a positive result in IHC in the following FFPE tissue: Human Colon Adenocarcinoma. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab107129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
  • 組織特異性Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
  • 配列類似性Belongs to the mitofusin family.
  • 翻訳後修飾Ubiquitinated by MARCH5.
  • 細胞内局在Cytoplasm and Mitochondrion outer membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Fzo homolog antibody
    • Hfzo1 antibody
    • Hfzo2 antibody
    • MFN 1 antibody
    • Mfn1 antibody
    • MFN1_HUMAN antibody
    • Mitochondrial transmembrane GTPase Fzo 1 antibody
    • Mitochondrial transmembrane GTPase FZO 2 antibody
    • Mitochondrial transmembrane GTPase FZO1B antibody
    • Mitofusin 1 antibody
    • Mitofusin-1 antibody
    • Mitofusin1 antibody
    • MS996 antibody
    • Putative transmembrane GTPase antibody
    • Transmembrane GTPase MFN1 antibody
    see all

Anti-Mitofusin 1 antibody 画像

  • ICC/IF image of ab107129 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107129 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken (ab96947) IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of Mitofusin 1 staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107129, 5µg/ml, for 15 mins at room temperature. A Goat anti-Chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Mitofusin 1 antibody (ab107129) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Kidney (Rat) Tissue Lysate
    Lane 4 : Human heart tissue lysate - total protein (ab29431)
    Lane 5 : Heart (Mouse) Tissue Lysate
    Lane 6 : Heart (Rat) Tissue Lysate
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
    Additional bands at : 17 kDa,27 kDa,36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-Mitofusin 1 antibody (ab107129) 使用論文

ab107129 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"