製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Mitofusin 1
  • アプリケーション
    適用あり: IP, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 683-732 (QVDITQKQLE EEIARLPKEI DQLEKIQNNS KLLRNKAVQL ENELENFTKQ) of Human Mitofusin 1 (NP_284941).

  • ポジティブ・コントロール
    • Human placenta lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
  • 組織特異性
    Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
  • 配列類似性
    Belongs to the mitofusin family.
  • 翻訳後修飾
    Ubiquitinated by MARCH5.
  • 細胞内局在
    Cytoplasm and Mitochondrion outer membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Fzo homolog antibody
    • Hfzo1 antibody
    • Hfzo2 antibody
    • MFN 1 antibody
    • Mfn1 antibody
    • MFN1_HUMAN antibody
    • Mitochondrial transmembrane GTPase Fzo 1 antibody
    • Mitochondrial transmembrane GTPase FZO 2 antibody
    • Mitochondrial transmembrane GTPase FZO1B antibody
    • Mitofusin 1 antibody
    • Mitofusin-1 antibody
    • Mitofusin1 antibody
    • MS996 antibody
    • Putative transmembrane GTPase antibody
    • Transmembrane GTPase MFN1 antibody
    see all

画像

  • Anti-Mitofusin 1 antibody (ab104274) at 1 µg/ml + Human placenta lysate at 10 µg

    Predicted band size : 84 kDa
  • All lanes : Anti-Mitofusin 1 antibody (ab104274) at 1/1000 dilution (+ 5% non-fat milk for 16 hours at 4°C )

    Lane 1 : Whole tissue lysate of mouse heart (wild type)
    Lane 2 : Whole tissue lysate of mouse heart (wild type)
    Lane 3 : Whole tissue lysate of mouse heart (Knock-out)
    Lane 4 : Whole tissue lysate of mouse heart (Knock-out)

    Lysates/proteins at 20 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 84 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at room temperature

参考文献

This product has been referenced in:
  • Yu J  et al. Sevoflurane postconditioning attenuates cardiomyocyte hypoxia/reoxygenation injury via restoring mitochondrial morphology. PeerJ 4:e2659 (2016). Rat . Read more (PubMed: 27833818) »
  • Mourier A  et al. Mitofusin 2 is required to maintain mitochondrial coenzyme Q levels. J Cell Biol 208:429-42 (2015). WB ; Mouse . Read more (PubMed: 25688136) »

See all 8 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - other (Mitochondrial Isolate)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
20 µg
Specification
Mitochondrial Isolate
Blocking step
Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Nov 22 2016

I believe there has been a misunderstanding here.

We try to provide all the information which we have regarding any product we carry and provide the most thorough data we can on our datasheets as you have undoubtably seen when using our Mito...

Read More

Thank you very much for your interest in our Mitofusin 1 antibodies.

Unfortunately the exact sequences of many of the immunogens used to create our proprietary. I have BLAST-ed the human and mouse Mitofusin 1 sequences and found that the ide...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse heart)
Loading amount
20 µg
Specification
mouse heart
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Oct 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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