1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 5 µg/ml.
Mitoferrin1, SLC25A37, belongs to the mitochondrial carrier family and contains 3 Solcar repeats. It is a mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells and plays an essential role in heme biosynthesis.
Mitochondrial inner membrane; Multi-pass membrane protein
ICC/IF image of ab102959 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab102959 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-Mitoferrin1 antibody (ab102959)
Anti-Mitoferrin1 antibody (ab102959) at 1/100 dilution + MDA-MB231 cell line lysate at 35 µg