A synthetic peptide corresponding to a region within internal sequence amino acids 229-278 (AVQGGKALMC VLRALSKNEE KAYKETQETQ ERDTLNKDHG NDKESNVLHQ) of human Mitochondrial Translational Initiation Factor 3 (NP_690876).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能IF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins.