Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 抗体 [4A11] (ab110025)

製品の概要

  • 製品名Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [4A11]
    Mitochondrial Pyruvate dehydrogenase kinase 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4A11] to Mitochondrial Pyruvate dehydrogenase kinase 1
  • アプリケーション適用あり: WB, ICC/IF, IHC-P, ELISA, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Monkey
  • 免疫原

    Recombinant fragment corresponding to Human Mitochondrial Pyruvate dehydrogenase kinase 1.

  • ポジティブ・コントロール
    • NIH3T3, Hela, Jurkat, HepG2, PC-12, and Cos7 cell lysate, human breast cancer and Human brain tissues, HELA and Lovo cells

アプリケーション

Our Abpromise guarantee covers the use of ab110025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Detects a band of approximately 44 kDa (predicted molecular weight: 49 kDa).
ICC/IF 1/200 - 1/1000.
IHC-P 1/200 - 1/1000.
ELISA 1/10000.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [4A11] 画像

  • All lanes : Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [4A11] (ab110025) at 1/500 dilution

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : PC-12 cell lysate
    Lane 6 : Cos7 cell lysate


    Predicted band size : 49 kDa
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using ab110025 aty a dilution of 1/200 with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded Human brain tissues using ab110025 aty a dilution of 1/200 with DAB staining.
  • Immunofluorescence analysis of HELA cells using ab110025 at a dilution of 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of Lovo cells using ab110025 (green) and negative control (purple).

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [4A11] (ab110025) 使用論文

This product has been referenced in:
  • Vavrova E  et al. Muscle expression of a malonyl-CoA-insensitive carnitine palmitoyltransferase-1 protects mice against high-fat/high-sucrose diet-induced insulin resistance. Am J Physiol Endocrinol Metab 311:E649-60 (2016). Read more (PubMed: 27507552) »
  • Wang LY  et al. KDM4A Coactivates E2F1 to Regulate the PDK-Dependent Metabolic Switch between Mitochondrial Oxidation and Glycolysis. Cell Rep 16:3016-27 (2016). Read more (PubMed: 27626669) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - other (muscle and liver)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
Loading amount 30 µg
Specification muscle and liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jun 30 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Mouse Cell (Liver mouse tissue)
Specification Liver mouse tissue
Permeabilization No
Fixative Methanol
Username

Dr. Dimitra Kalamida

Verified customer

投稿 Dec 09 2013

Thank you for contacting us.

As always, if we do not advise a certain method on our datasheet, the customer can use the one which they usually use within their lab. This is the case of ab110025.

For ab65979 I would suggest checking th...

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Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from these antibodies.

The details your customer has kindly provi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"