Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 抗体 [2H3AA11] (ab110335)

製品の概要

  • 製品名Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [2H3AA11]
    Mitochondrial Pyruvate dehydrogenase kinase 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2H3AA11] to Mitochondrial Pyruvate dehydrogenase kinase 1
  • 特異性This antibody does not cross-react with other isoforms of Human PDK (i.e. PDK2, 3 or 4) and does not cross-react with tissue culture lysates, Rat, or Mouse samples
  • アプリケーション適用あり: ICC/IF, In-Cell ELISA, WB, Flow Cytmore details
  • 種交差性
    交差種: Cow, Human
  • 免疫原

    Recombinant human PDK1

  • ポジティブ・コントロール
    • HeLa cells; Human heart and liver tissue lysates; and HL-60 cells
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
In-Cell ELISA Use a concentration of 2 µg/ml. 0.2 µg/well
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • 組織特異性Expressed predominantly in the heart.
  • 配列類似性Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • 細胞内局在Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody
    • HGNC:8809 antibody
    • Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody
    • PDH kinase 1 antibody
    • Pdk1 antibody
    • PDK1_HUMAN antibody
    • Pyruvate dehydrogenase kinase isoform 1 antibody
    • Pyruvate dehydrogenase kinase, isoenzyme 1 antibody
    see all

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [2H3AA11] 画像



  • Predicted band size : 49 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Mitochondrial Pyruvate dehydrogenase kinase 1 knockout HAP1 cell lysate (20 µg)
    Lane 3: Human fetal heart lysate (20 µg)
    Lane 4: LnCaP cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab110335 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab110335 was shown to recognize Mitochondrial Pyruvate dehydrogenase kinase 1 when Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were subjected to SDS-PAGE. ab110335 and ab8245 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [2H3AA11] (ab110335) at 1 µg/ml

    Lane 1 : Human heart lysate at 10 µg
    Lane 2 : Human liver lysate at 10 µg
    Lane 3 : Recombinant Human PDK1 at 0.01 µg
    Lane 4 : Recombinant Human PDK2 at 0.01 µg
    Lane 5 : Recombinant Human PDK3 at 0.01 µg
    Lane 6 : Recombinant Human PDK4 at 0.01 µg


    Predicted band size : 49 kDa
  • All lanes : Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [2H3AA11] (ab110335) at 1 µg/ml

    Lane 1 : HeLa cells incubated for 24 hours with vehicle
    Lane 2 : HeLa cells incubated for 24 hours with 100 µM cobalt chloride (CoCl2)
    Lane 3 : HeLa cells incubated for 24 hours with 1 mM deferoxamine (DFO)

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 49 kDa
    Treatment with either CoCl2 or DFO results in increased expression of PDK1, which is known to be transcriptionally upregulated in response to hypoxia.
  • HL-60 cells were stained with 1 µg/mL PDK1 antibody (ab110335) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunocytochemistry image of PDK1 stained HeLa cells. The Upper image shows cells which were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the PDK1 antibody (ab110335) at 2 µg/mL for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.
    The lower image shows cells co-stained with an antibody against HSP60 (red), an enzyme also located in the mitochondrial matrix. The composite image shows an identical mitochondrial pattern for both antibodies indicated by merged orange color.

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [2H3AA11] (ab110335) 使用論文

This product has been referenced in:
  • Goto M  et al. Adaptation of leukemia cells to hypoxic condition through switching the energy metabolism or avoiding the oxidative stress. BMC Cancer 14:76 (2014). WB ; Human . Read more (PubMed: 24506813) »

See 1 Publication for this product

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