リコンビナント
RabMAb

Anti-Mitochondrial Ferritin 抗体 [EPR1797] (ab124889)

製品の概要

  • 製品名
    Anti-Mitochondrial Ferritin antibody [EPR1797]
    Mitochondrial Ferritin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1797] to Mitochondrial Ferritin
  • アプリケーション
    適用あり: WBmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Mitochondrial Ferritin aa 200-300 (C terminal).
    Database link: Q8N4E7

  • ポジティブ・コントロール
    • Human Testis lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 28 kDa).
  • 追加情報
    Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 機能
      Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.
    • 組織特異性
      Detected in testis and erythroleukemia. Expression is very low or not detectable in brain, colon, heart, kidney, liver, lung, muscle, placental, spleen and small intestine.
    • 配列類似性
      Belongs to the ferritin family.
      Contains 1 ferritin-like diiron domain.
    • 細胞内局在
      Mitochondrion.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FerH antibody
      • Ferritin antibody
      • Ferritin H subunit antibody
      • Ferritin, mitochondrial antibody
      • Fth 3 antibody
      • Fth3 antibody
      • FTMT antibody
      • FTMT_HUMAN antibody
      • mitochondrial antibody
      • Mitochondrial ferritin antibody
      • MTF antibody
      • OTTHUMP00000159158 antibody
      see all

    画像

    • Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889) at 1/1000 dilution + Human Testis lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 28 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    プロトコール

    参考文献

    This product has been referenced in:
    • Shi ZH  et al. Mitochondrial ferritin, a new target for inhibiting neuronal tumor cell proliferation. Cell Mol Life Sci N/A:N/A (2014). WB . Read more (PubMed: 25213357) »

    See 1 Publication for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HepG2)
    Loading amount
    50 µg
    Specification
    HepG2
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jul 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録