Anti-Mitochondrial Ferritin 抗体 (ab93428)

製品の概要

  • 製品名Anti-Mitochondrial Ferritin antibody
    Mitochondrial Ferritin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Mitochondrial Ferritin
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human Mitochondrial Ferritin aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q8N4E7
    (Peptide available as ab93799)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Hela and HEK293 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 27 kDa).

ターゲット情報

  • 機能Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.
  • 組織特異性Detected in testis and erythroleukemia. Expression is very low or not detectable in brain, colon, heart, kidney, liver, lung, muscle, placental, spleen and small intestine.
  • 配列類似性Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • 細胞内局在Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FerH antibody
    • Ferritin antibody
    • Ferritin H subunit antibody
    • Ferritin, mitochondrial antibody
    • Fth 3 antibody
    • Fth3 antibody
    • FTMT antibody
    • FTMT_HUMAN antibody
    • mitochondrial antibody
    • Mitochondrial ferritin antibody
    • MTF antibody
    • OTTHUMP00000159158 antibody
    see all

Anti-Mitochondrial Ferritin antibody 画像

  • All lanes : Anti-Mitochondrial Ferritin antibody (ab93428) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
    The band observed at 22 kDa could potentially be a cleaved form of Human Ferritin, mitochondrial precursor due to a 49 amino acid transit peptide (SwissProt), which when cleaved gives an expected Mw of 22 kDa.

Anti-Mitochondrial Ferritin antibody (ab93428) 使用論文

ab93428 has not yet been referenced specifically in any publications.

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