製品の概要

  • 製品名Mitochondrial DNA Isolation Kit
  • テスト
    50 x 1 assay
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • アッセイタイプDirect
  • 製品の概要

    Abcam's Mitochondrial DNA Isolation Kit provides convenient tools for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies such as enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Mitochondria are semi-autonomous organelles which functions in aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer's disease, and muscle disorders. Isolation and quantification of mtDNA are often required to study the relationships between the diseases and mtDNA.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Mitochondria are semiautonomous organelles which function in the aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer’s disease, and muscle disorders.

アプリケーション

Our Abpromise guarantee covers the use of ab65321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

プロトコール

Mitochondrial DNA Isolation Kit (ab65321) 使用論文

This product has been referenced in:
  • Martin SA  et al. Parallel high-throughput RNA interference screens identify PINK1 as a potential therapeutic target for the treatment of DNA mismatch repair-deficient cancers. Cancer Res 71:1836-48 (2011). Read more (PubMed: 21242281) »
  • Munwes I  et al. Variation in repeat length and heteroplasmy of the mitochondrial DNA control region along a core-edge gradient in the eastern spadefoot toad (Pelobates syriacus). Mol Ecol 20:2878-87 (2011). Read more (PubMed: 21645158) »

See all 3 Publications for this product

Product Wall



I can confirm there is RNase contained in the mitochondrial lysis buffer.

This is RnaseA from Bovine pancreas.

Thank you for contacting Abcam regarding ab65321.


Regarding this Mitochondrial DNA Isolation Kit:


1 - Expect to recover 5-20ug mtDNA

2- The most important step with this kit is to isolate a pure mitochondrial ...

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Mitochondrial DNA isolation

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Mitochondrial DNA (mtDNA) isolation has been performed using mitochondrial DNA Isolation Kit (Abcam, ab65321) following the manufacturer’s instructions. MtDNA has been isolated by MRC5 fibloblasts in high yield and purity, without contaminations from genomic DNA. 2.22 x 106 cells have been used in each condition, the concentration of the purified mtDNA was then measured using NanoDrop 2000, and graph presentation has been performed using the GraphPad Prism Version 5.01a statistical package (GraphPad Software Inc., USA). A 1% agarose gel stained with EtBr was also run to confirm the correct size (~ 17kB) using a DNA ladder, the purity and the concentration of mtDNA, and it was imaged using Chemidoc MP Imaging System (Biorad, USA).
Personal feedback: Mitochondrial DNA isolation Kit can be safely used to isolate mtDNA in high yield and purity, by using a standard cell number in each condition, the effect of various treatments in the number of mitochondria can be easily measured.
Username

Dr. Dimitra Kalamida

Verified customer

投稿 Nov 10 2016

Extraction of mtDNA from whole blood

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This is a great kit to extract mitochondrial DNA. We just added an extra step of isolating Peripheral Blood Mononuclear Cells (PBMC) from whole blood and applied the abcam protocol (with minor changes, see attached protocol) to isolate mtDNA from PBMC.
Username

Lina Abi Mosleh

Verified customer

投稿 Apr 20 2016

No, the Enzyme B Mix or any other component should not neutralize ROS species.

ab65321 is designed to isolate mitochondria first and then lyse the mitochondria to get the mtDNA. Thus, since you have a solution of DNA with genomic and mtDNA mixed in, then this kit is not designed to isolate the mtDNA specifically. One way the lab ...

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Both the Lysis buffer and Enzyme mix are available separately. Here are the product numbers:

Lysis Buffer - ab192492 - http://www.abcam.com/mitochondrial-lysis-buffer-ab192492.html

Enzyme Mix - ab186679 http://www.abcam.com/enzyme-b-m...

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Thank you for your enquiries regarding ab65321 Mitochondrial DNA Isolation Kit .

I have now obtained some further information from the laboratories.

As I thought, the Enzyme B composition is proprietary. However, I can confirm there ...

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I can confirm that the isolated mtDNA in the mitochondria isolated with this kit will be circular and will run at ˜15-20 kbs.

Vielen Dank für Ihre Geduld.

Leider ist diese Information ein Betriebsgeheimnis, doch das Labor ist gerne bereit, auf mehr spezielle Fragen einzugehen, wie zb. pH- Wert einzelner Reagenzien, oder Verwendung nach der Lyse.

Ich...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"