Anti-Mitochondrial dicarboxylate carrier 抗体 (ab32632)
Key features and details
- Rabbit polyclonal to Mitochondrial dicarboxylate carrier
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Mitochondrial dicarboxylate carrier antibody
Mitochondrial dicarboxylate carrier 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Mitochondrial dicarboxylate carrier -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide: LRKHFGIKVP TT, corresponding to C terminal amino acids 276-287 of Mouse Mitochondrial dicarboxylate carrier, extracted from mouse fibroblast.
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ポジティブ・コントロール
- WB: 3T3-L1 (Mouse embryonic fibroblast cell line) whole cell lysate differentiated to adipocytes ; Mouse white adipose ; Mouse Liver ; Mouse Kidney ICC/IF: C2C12 (Mouse myoblast cell line) whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32632の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500. Predicted molecular weight: 31 kDa.
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ICC/IF |
1/50 - 1/500.
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特記事項 |
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WB
1/500. Predicted molecular weight: 31 kDa. |
ICC/IF
1/50 - 1/500. |
ターゲット情報
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関連性
The mitochondrial dicarboxylate carrier protein (mDIC) belongs to superfamily of mitochondrial transporters. It has been demonstrated that the expression of mDIC leads to hyperpolarization of the mitochondria, as well as succinate uptake by the mitochondria. It is highly expressed in adipose tissue, where its expression is downregulated by insulin and upregulated by free fatty acids. mDIC is also thought to play a role in glyceroneogenesis. -
細胞内局在
Mitochondrion; mitochondrial inner membrane; multi pass membrane protein. -
参照データベース
- Entrez Gene: 27376 Mouse
- SwissProt: Q543J6 Mouse
- SwissProt: Q9D817 Mouse
- SwissProt: Q9QZD8 Mouse
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別名
- DIC antibody
- SLC25A10 antibody
- Solute carrier family 25 member 10 antibody
画像
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All lanes : Anti-Mitochondrial dicarboxylate carrier antibody (ab32632) at 1/500 dilution
Lane 1 : 3T3-L1 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : 3T3-L1 (Mouse embryonic fibroblast cell line) whole cell lysate differentiated to adipocytes
Lane 3 : Mouse white adipose
Lane 4 : Mouse Liver
Lane 5 : Mouse Kidney
Lane 6 : Mouse Spleen
Lane 7 : Mouse Brain
Lane 8 : Mouse Skeletal Muscle
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
Predicted band size: 31 kDa
Additional bands at: ~31 kDa. We are unsure as to the identity of these extra bands.Antibody specificity demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Mitochondrial dicarboxylate carrier was observed in Mouse White Adipose, Mouse Liver, Mouse Kidney compared to other tissue extracts tested and was also enhanced in 3T3-L1 (Mouse embryonic fibroblast cell line) whole cell lysate after differentiating it to adipocytes.
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Immunofluorescent analysis of Mitochondrial dicarboxylate carrier (Green) labeling C2C12 (Mouse myoblast cell line) whole cell lysate. The cells were formalin-fixed and permeabilized with 0.1% Triton™ X-100 in TBS for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. The cells were labeled with ab32632 at a dilution of 1/100 in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei (Blue) were stained with DAPI. F-actin (Red) was stained with Alexa Fluor® 554. Left image is a no primary antibody control. The images were captured at 60X magnification.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab32632 は論文での使用が確認できていません。