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ab110169 (MS851) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Tested tissues: brain, liver and heart
|Dounce Homogenizer (2 ml size) with pestles||1 unit|
|Isolation Buffer||1 x 100ml|
|Wash Buffer||1 x 30ml|
Our Abpromise guarantee covers the use of ab110169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution. Mitochondria isolations from hard or soft tissues.|
Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 µg (lane 1) and 10 µg (lane 2) of mitochondria were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4