Anti-Mitochondria 抗体 [SPM198], prediluted (ab17857)

Submit an Abreview Q&A (2)Specific References (2)

製品の概要

  • 製品名
    Anti-Mitochondria antibody [SPM198], prediluted
    Mitochondria 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SPM198] to Mitochondria, prediluted
  • 由来種
    Mouse
  • 特異性
    This antibody recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Semi-purified mitochondrial preparation

  • ポジティブ･コントロール
    • Liver
  • 特記事項


    ab17857 recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in human cell or tissue preparations and can be used as a mitochondrial-marker in subcellular fractions. ab17857 also detects the organelles within mitochondria; characteristic ('spaghetti'-like) mitochchondrial staining pattern is observed in the cytoplasm of normal and malignant human cells.

製品の特性

  • 製品の状態
    Prediluted
  • 保存方法
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 50mM Tris
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 一次抗体 備考
    ab17857 recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in human cell or tissue preparations and can be used as a mitochondrial-marker in subcellular fractions. ab17857 also detects the organelles within mitochondria; characteristic ('spaghetti'-like) mitochchondrial staining pattern is observed in the cytoplasm of normal and malignant human cells.
  • ポリ/モノ
    モノクローナル
  • クローン名
    SPM198
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab17857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Ready-to-use for 30 min at RT.

ターゲット情報

  • 関連性
    Mitochondria are the power house of the cell. They are distinct organelles with two membranes. Usually they are rod shaped, however they can be round. The outer membrane limits the organelle and the inner membrane is thrown into folds or shelves that project inward and are called "cristae mitochondriales".
  • 細胞内局在
    Mitochondrial

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [SPM198], prediluted (ab17857)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [SPM198], prediluted (ab17857)
    ab17857 detecting mitochondria in human liver (dilution of ab17857 =  ready-to-use for 30min at RT)

プロトコール

参考文献

This product has been referenced in:
  • Pusiol T  et al. Value of imaging and aspiration cytology in the diagnosis of oncocytic carcinoma. Acta Otorhinolaryngol Ital 30:110-4 (2010). Read more (PubMed: 20559483) »
  • Lévesque MH  et al. Androgen receptor as a potential sign of prostate cancer metastasis. Prostate 69:1704-11 (2009). IHC-P ; Human . Read more (PubMed: 19670238) »

See all 2 Publications for this product

ab17857 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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2 reviews or Q&A

10
Votes: Highest First

Dear Abcam team,
We are looking for a way to distinguish between mouse and human cells
following orthotopic injection of tumorigenic prostate cells into the
mouse prostate. The resulting tumor is fixed in formaldehyde and
paraffin e...

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Thank you for contacting us.

Regarding antibody ab17857 I am afraid we have not tested it with mouse samples, therefore the cross-reactivity with this species is not known.

We have other antibodies against Mitochondria validated in ...

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Dear Sir/Madam The customer do the research by insert human tissue into node mouse. So, she would like to know the antibodies that not react with mouse. Pleases kindly provide information. ANTI-MITOCHONDRIA ANTIBODY [MTC02] - MITOCHONDRIAL MARKER (AB32...

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Thank you for your enquiry and interest. As the datasheets indicate these antibodies have been tested for cross-reaction with human but not data available for mouse. All of these three antibodies are raised in mouse, so high non-specific background cou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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