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Semi-purified mitochondrial preparation.
ab79479 produces a spaghetti like staining pattern in the cytoplasm of normal and malignant human cells.
Our Abpromise guarantee covers the use of ab79479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent dilution.|
ab79479 staining Mitochondria in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with antibody block for 10 minutes; antigen retrieval was by heat mediation in CC1 Mild. Samples were incubated with primary antibody (1/100 in antibody diluent) for 1 hour at 37°C. An undiluted Biotin-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.
ab79479 has not yet been referenced specifically in any publications.