Anti-Mitochondria 抗体 [MTC02] (Biotin) (ab79479)

製品の概要

  • 製品名
    Anti-Mitochondria antibody [MTC02] (Biotin)
    Mitochondria 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MTC02] to Mitochondria (Biotin)
  • 標識
    Biotin
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Semi-purified mitochondrial preparation.

  • ポジティブ・コントロール
    • HeLa cells. Liver.
  • 特記事項


    ab79479 produces a spaghetti like staining pattern in the cytoplasm of normal and malignant human cells.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 関連性
    Mitochondria are the power house of the cell. They are distinct organelles with two membranes. Usually they are rod shaped, however they can be round. The outer membrane limits the organelle and the inner membrane is thrown into folds or shelves that project inward and are called "cristae mitochondriales".
  • 細胞内局在
    Mitochondrial

画像

  • ab79479 staining Mitochondria in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with antibody block for 10 minutes; antigen retrieval was by heat mediation in CC1 Mild. Samples were incubated with primary antibody (1/100 in antibody diluent) for 1 hour at 37°C. An undiluted Biotin-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • IHC image of ab79479 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79479, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

ab79479 has not yet been referenced specifically in any publications.

レビューと Q&A

The ab79479 Anti-Mitochondria antibody [MTC02] (Biotin) has the clone name MTCO2. The antibody does detect a mitochondrial protein but I can confirm this is not MTCO2. The clone name is a coincidence.

We do have antibodies for specific detecti...

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Ventana Antibody Block as blocking agent for 10 minute(s) · Concentration: 100%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1 Mild
Sample
Human Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
10% NBF
Username

Abcam user community

Verified customer

投稿 Dec 16 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney and Spleen)
Specification
Kidney and Spleen
Fixative
10% NBF
Antigen retrieval step
Other
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Feb 13 2013

Thank you for your enquiry and interest. As the datasheets indicate these antibodies have been tested for cross-reaction with human but not data available for mouse. All of these three antibodies are raised in mouse, so high non-specific background cou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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